Yang Liyuan, Zheng Weiwei, Xin Shiying, Lv Xing, Sun Yuena, Xu Tianjun
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China.
Fish Shellfish Immunol. 2022 Mar;122:345-351. doi: 10.1016/j.fsi.2022.02.025. Epub 2022 Feb 17.
The inhibitory protein IκBα plays a key role in the inflammatory process and immune response by regulating the activity of the transcription factor NF-κB. microRNA (miR) is a small non-coding RNA that can regulate many biochemical processes, such as cell growth, proliferation, and immune response. In this study, it was first predicted that IκBα is the target of miR-122 through bioinformatics, and it was confirmed by dual fluorescence experiments. Then we found that miR-122 can inhibit the expression of IκBα at the mRNA and protein levels, thereby promoting the p65-activated NF-κB pathway. It is speculated that miR-122 plays an important role in the innate immunity of teleost fish. This study will help to further understand miRNAs regulatory mechanism in teleost fish.
抑制蛋白IκBα通过调节转录因子NF-κB的活性在炎症过程和免疫反应中起关键作用。微小RNA（miR）是一种小的非编码RNA，可调节许多生化过程，如细胞生长、增殖和免疫反应。在本研究中，首先通过生物信息学预测IκBα是miR-122的靶标，并通过双荧光实验得到证实。然后我们发现miR-122可在mRNA和蛋白质水平抑制IκBα的表达，从而促进p65激活的NF-κB途径。推测miR-122在硬骨鱼的先天免疫中起重要作用。本研究将有助于进一步了解硬骨鱼中miRNA的调控机制。
