Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, 712100, China; Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, 712100, China; Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Mol Cell Endocrinol. 2022 Dec 1;558:111765. doi: 10.1016/j.mce.2022.111765. Epub 2022 Aug 29.
Estradiol (E) synthesis, cell proliferation and the apoptosis of porcine granulosa cells (GCs) affect follicular growth and development. The miR-184 level in ovary tissues of Yorkshire × Landrace sows was significantly higher in high-yielding sows than that in low-yielding sows, which was the same as in Yorkshire sows. However, the roles of miR-184 on E granulosa cells (GCs) are still unclear. We found that miR-184 promoted E synthesis and proliferation but inhibited apoptosis in GCs by targeting nuclear receptor subfamily 1 group D member 1 (NR1D1), cyclin dependent kinase inhibitor 1A (P21,CDKN1A) and homeodomain interacting protein kinase 2 (HIPK2) respectively. These findings indicated that miR-184 is a novel key factor that regulates the physiological functions of GCs.
雌二醇（E）的合成、细胞增殖以及猪颗粒细胞（GCs）的凋亡影响卵泡的生长和发育。在高产约克夏×长白母猪的卵巢组织中，miR-184 的水平明显高于低产母猪，与约克夏母猪相同。然而，miR-184 对 E 颗粒细胞（GCs）的作用尚不清楚。我们发现，miR-184 通过靶向核受体亚家族 1 组 D 成员 1（NR1D1）、细胞周期蛋白依赖性激酶抑制剂 1A（P21、CDKN1A）和同源结构域相互作用蛋白激酶 2（HIPK2）分别促进 GCs 中 E 的合成和增殖，同时抑制其凋亡。这些发现表明，miR-184 是调节 GCs 生理功能的一个新的关键因素。
