Medical College, Xinxiang University, Xinxiang, China.
School of Life Science and Basic Medicine, Xinxiang University, Xinxiang, China.
Microb Pathog. 2022 Oct;171:105744. doi: 10.1016/j.micpath.2022.105744. Epub 2022 Aug 30.
Type III secretion system 1 (T3SS1) encoded by Salmonella pathogenicity island 1 (SPI1) is associated with invasion of host cells by Salmonella, PrgH encoded by prgH gene is an important component of T3SS1. This study aimed to explore the contribution of prgH gene for Salmonella Pullorum to virulence and the expression of NLRP3, Caspase-1 and IL-1β in chickens. A prgH gene deletion mutant (C79-13ΔprgH) was firstly generated, and the result of LD showed that deletion of prgH significantly decreased the virulence of Salmonella Pullorum in one-day-old HY-line white chickens, and the colonization also decreased in chickens after loss of prgH. Next, the expressions of NLRP3, Caspase-1, and IL-1β were detected in acute infection model of chickens by qRT-PCR and/or ELISA, respectively, and the results showed that the mutant strain C79-13ΔprgH reduced the expression levels of NLRP3, Caspase-1, and IL-1β in chickens compared to the group infected with the wild type strain C79-13. Taken together, all of these findings indicated that prgH promotes the virulence and the expression of NLRP3, Caspase-1, and IL-1β for Salmonella Pullorum in chickens.
III 型分泌系统 1（T3SS1）由沙门氏菌致病性岛 1（SPI1）编码，与沙门氏菌入侵宿主细胞有关，PrgH 基因编码的 PrgH 是 T3SS1 的重要组成部分。本研究旨在探讨 prgH 基因对鸡沙门氏菌肠炎沙门氏菌毒力的贡献，以及 NLRP3、Caspase-1 和 IL-1β在鸡中的表达。首先生成了 prgH 基因缺失突变体（C79-13ΔprgH），LD 结果表明，prgH 的缺失显著降低了鸡沙门氏菌肠炎沙门氏菌在 1 日龄 HY 系白鸡中的毒力，缺失 prgH 后鸡的定植也减少。接下来，通过 qRT-PCR 和/或 ELISA 分别检测鸡急性感染模型中 NLRP3、Caspase-1 和 IL-1β的表达，结果表明突变株 C79-13ΔprgH 与感染野生型菌株 C79-13 的组相比，降低了 NLRP3、Caspase-1 和 IL-1β在鸡中的表达水平。综上所述，这些发现表明 prgH 促进了鸡沙门氏菌肠炎沙门氏菌的毒力和 NLRP3、Caspase-1 和 IL-1β的表达。
