National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China; Beijing Environmental Engineering Technology Co. Ltd., PR China.
Bioresour Technol. 2022 Nov;363:127865. doi: 10.1016/j.biortech.2022.127865. Epub 2022 Aug 30.
This study established the one-stage partial nitrification coupled anammox and partial denitrification coupled anammox process in an anoxic/oxic continuous plug-flow system and operated for 465 days to treat mature landfill leachate. 97.9 %-98.1 % of inorganic nitrogen was removed when the nitrogen loading rate was maintained at 0.33-0.36 kg N/m/d, and a high anammox contribution to nitrogen removal (89.8 %-92.4 %) was achieved. The long-term in-situ free ammonia (FA) anoxic treatment contributed to the stable performances of partial nitrification and in-situ fermentation. The employed integrated fixed-film activated sludge technology favored the enrichment of hzsA, hzsB, hdh, amoA, hao, narG, and napA functional genes. The oxic zone, particularly oxic biofilm, was the key zone for anammox pathway, where Candidatus_Kuenenia (from 1.6 % to 8.3 %) with high tolerance to FA and salinity stress outcompeted Candidatus_Brocadia (from 18.3 % to 0.1 %) as the dominant anammox bacteria. This study could provide guidance for anammox-mediated landfill leachate treatment in practical projects.
本研究在缺氧/好氧连续推流式系统中建立了单级部分硝化耦合厌氧氨氧化和部分反硝化耦合厌氧氨氧化工艺，并运行了 465 天来处理成熟的垃圾渗滤液。当氮负荷保持在 0.33-0.36 kg N/m/d 时，可去除 97.9%-98.1%的无机氮，实现了较高的厌氧氨氧化对氮去除的贡献（89.8%-92.4%）。长期原位游离氨（FA）缺氧处理有助于部分硝化和原位发酵的稳定运行。所采用的集成固定膜活性污泥技术有利于 hzsA、hzsB、hdh、amoA、hao、narG 和 napA 功能基因的富集。好氧区，特别是好氧生物膜，是厌氧氨氧化途径的关键区，其中对 FA 和盐度胁迫具有高耐受性的卡氏菌（从 1.6%增加到 8.3%）竞争胜过布鲁卡氏菌（从 18.3%减少到 0.1%），成为优势厌氧氨氧化菌。本研究可为实际工程中厌氧氨氧化介导的垃圾渗滤液处理提供指导。
