State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Oral Dis. 2024 Mar;30(2):650-659. doi: 10.1111/odi.14365. Epub 2022 Sep 12.
This study aims to clarify the effects of diabetes mellitus (DM) on inflammatory profile during orthodontic tooth movement (OTM) and explore potential mechanisms.
OTM models were established in healthy (Ctrl) and DM rats for 0, 3, 7 or 14 days. The tooth movement distance and bone structural parameters were analyzed through micro-CT. The bone resorption activity and periodontal inflammation status were evaluated through histological staining. RNA sequencing was performed to detect differentially expressed genes in force loading-treated periodontal ligament fibroblasts (PDLFs) with or without high glucose. The differential expression of inflammatory genes associated with NOD-like receptor family pyrin domain containing 3 (NLRP3) between groups was tested in vitro and in vivo.
DM caused remarkable reduction of alveolar bone height and density around the moved tooth, corresponding with the higher bone resorption activity and inflammatory scores of DM group. For force loading-treated PDLFs, high glucose induced the activation of inflammatory pathways, including NLRP3. Elevated expression of NLRP3 and cascade molecules (Caspase-1, GSDMD, and IL-1β) were validated by RT-qPCR, Western blot, and immunohistochemistry staining.
DM alters the inflammatory status of periodontium and affects tissue reconstruction during OTM. NLRP3 inflammasome may involve in diabetes-induced periodontal changes.
本研究旨在阐明糖尿病（DM）对正畸牙齿移动（OTM）过程中炎症谱的影响，并探讨潜在机制。
建立健康（Ctrl）和 DM 大鼠的 OTM 模型，分别于 0、3、7 或 14 天进行检测。通过 micro-CT 分析牙齿移动距离和骨结构参数。通过组织学染色评估骨吸收活性和牙周炎症状态。通过 RNA 测序检测力加载处理的牙周膜成纤维细胞（PDLFs）在高糖条件下差异表达的基因。在体外和体内检测各组间与 NOD 样受体家族富含 pyrin 域蛋白 3（NLRP3）相关的炎症基因的差异表达。
DM 导致牙槽骨高度和移动牙周围骨密度明显降低，相应的骨吸收活性和 DM 组的炎症评分较高。对于力加载处理的 PDLFs，高糖诱导炎症途径的激活，包括 NLRP3。通过 RT-qPCR、Western blot 和免疫组织化学染色验证 NLRP3 和级联分子（Caspase-1、GSDMD 和 IL-1β）的表达上调。
DM 改变牙周组织的炎症状态，并影响 OTM 过程中的组织重建。NLRP3 炎性小体可能参与糖尿病引起的牙周变化。
