Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, 2193, South Africa.
AAPS PharmSciTech. 2022 Sep 1;23(7):247. doi: 10.1208/s12249-022-02397-4.
Cytocompatibility, biocompatibility, and biodegradability are amongst the most desirable qualities of wound dressings and can be tuned during the bioplatform fabrication steps to enhance wound healing capabilities. A three-stepped approach (partial-crosslinking, freeze-drying, and pulverisation) was employed in fabricating a particulate, partially crosslinked (PC), and transferulic acid (TFA)-loaded chitosan-alginate (CS-Alg) interpolymer complex (IPC) with enhanced wound healing capabilities. The PC TFA-CS-Alg IPC bioplatform displayed fluid uptake of 3102% in 24 h and a stepwise degradation up to 53.5% in 14 days. The PC TFA-CS-Alg bioplatform was used as a bioactive delivery system with an encapsulation efficiency of 65.6%, bioactive loading of 9.4%, burst release of 58.27%, and a steady release of 1.91% per day. PC TFA-CS-Alg displayed a shift in cytocompatibility from slightly cytotoxic (60-90% cell viability) to nontoxic (> 90% cell viability) over a 72-h period in NIH-3T3 cells. The wound closure and histological evaluations of the lesions indicated better wound healing performance in lesions treated with PC TFA-CS-Alg and PC CS-Alg compared to those treated with the commercial product and the control. Application of the particulate bioplatform on the wound via sprinkles, the in situ hydrogel formation under fluid exposure, and the accelerated wound healing performances of the bioplatforms make it a good candidate for bioactive delivery system and skin tissue regeneration.
细胞相容性、生物相容性和生物降解性是伤口敷料最理想的特性之一，可以在生物平台制造步骤中进行调整，以增强伤口愈合能力。采用三步法（部分交联、冷冻干燥和粉碎）制备具有增强伤口愈合能力的颗粒状、部分交联（PC）和负载转移酸（TFA）的壳聚糖-海藻酸钠（CS-Alg）互聚物复合物（IPC）。PC TFA-CS-Alg IPC 生物平台在 24 小时内的吸水率为 3102%，并在 14 天内逐步降解至 53.5%。PC TFA-CS-Alg 生物平台用作具有封装效率为 65.6%、生物活性负载为 9.4%、突释释放 58.27%和每天 1.91%稳定释放的生物活性递送系统。PC TFA-CS-Alg 在 NIH-3T3 细胞中在 72 小时内显示出细胞相容性从轻微细胞毒性（60-90%细胞活力）转变为无毒（>90%细胞活力）。病变的伤口闭合和组织学评估表明，与商用产品和对照相比，用 PC TFA-CS-Alg 和 PC CS-Alg 处理的病变的伤口愈合性能更好。通过撒播将颗粒状生物平台应用于伤口，在暴露于流体时原位形成水凝胶，以及生物平台的加速伤口愈合性能，使其成为生物活性递送系统和皮肤组织再生的良好候选物。
