Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Wielkopolskie, Poland.
Plant Breeding and Acclimatization Institute - National Research Institute, Młochów, Masovian Voivodeship, Poland.
Physiol Plant. 2022 Sep;174(5):e13775. doi: 10.1111/ppl.13775.
Drought is one of the main climate threats limiting crop production. Potato is one of the four most important food crop species worldwide and is sensitive to water shortage. The CBP80 gene was shown to affect Arabidopsis and potato responses to drought by regulating the level of microRNA159 and, consequently, the levels of the MYB33 and MYB101 transcription factors (TFs). Here, we show that three MYB TFs, MYB33, MYB65, and MYB101, are involved in plant responses to water shortage. Their downregulation in Arabidopsis causes stomatal hyposensitivity to abscisic acid (ABA), leading to reduced tolerance to drought. Transgenic Arabidopsis and potato plants overexpressing these genes, with a mutated recognition site in miR159, show hypersensitivity to ABA and relatively high tolerance to drought conditions. Thus, the MYB33, MYB65, and MYB101 genes may be potential targets for innovative breeding to obtain crops with relatively high tolerance to drought.
干旱是限制作物生产的主要气候威胁之一。马铃薯是世界上四种最重要的粮食作物之一，对缺水敏感。CBP80 基因被证明通过调节 microRNA159 的水平，从而调节 MYB33 和 MYB101 转录因子（TFs）的水平，影响拟南芥和马铃薯对干旱的响应。在这里，我们表明三个 MYB TF，MYB33、MYB65 和 MYB101，参与植物对水分短缺的响应。它们在拟南芥中的下调导致气孔对脱落酸（ABA）的敏感性降低，导致对干旱的耐受性降低。过表达这些基因的拟南芥和马铃薯转基因植物，其 miR159 的识别位点发生突变，对 ABA 表现出超敏感性，并且对干旱条件具有相对较高的耐受性。因此，MYB33、MYB65 和 MYB101 基因可能是创新育种的潜在目标，以获得对干旱具有相对较高耐受性的作物。
