CRISPR/Cas9 editing of enhances drought tolerance in potato (Solanum tuberosum).

Developing drought-tolerant potato varieties is increasingly important due to climate change and water scarcity, as potatoes are highly sensitive to water deficits that can significantly reduce yield and tuber quality. The cap-binding protein CBP80, involved in the abscisic acid (ABA) signalling pathway, has emerged as a promising target for improving drought tolerance in plants. In this study, we used CRISPR/Cas9 to edit the gene in the tetraploid potato cultivar Spunta. Given the complexity of editing all four alleles in a tetraploid genome, eight independent partially edited lines (two or three alleles edited) were obtained. Two of these lines were selected for detailed molecular and phenotypic characterization. Under restricted water conditions, the selected lines exhibited reduced transpiration rates and improved leaf area index compared to non-edited controls. Gene expression analysis by quantitative real-time PCR showed differential expression of drought-responsive genes (P5CS, PDH, and ), supporting a role for in stress response modulation. Moreover, the edited lines showed lower yield penalties, both in biomass and tuber production, under drought stress. This work represents one of the first applications of genome editing to enhance drought tolerance in a commercial potato cultivar, and highlights CBP80 as a promising target for crop improvement. These findings provide valuable insights for the development of stress-resilient potato varieties using genome editing approaches.