Zhang Zhipeng, Wang Jinyao, Xing Guoming, Li Meilan, Li Sen
College of Horticulture, Shanxi Agricultural University, Jinzhong, China.
Collaborative Innovation Center for Improving Quality and Increase of Protected Vegetables in Shanxi Province, Jinzhong, China.
Front Plant Sci. 2022 Aug 16;13:972620. doi: 10.3389/fpls.2022.972620. eCollection 2022.
Chloroplasts are the material basis of photosynthesis, and temperature and light severely affect chloroplast development and thus influence photosynthetic efficiency. This study identified a spontaneous virescent leaf mutant, SC311Y, whose cotyledons and true leaves were yellow and gradually turned green. However, temperature and light affected the process of turning green. In addition, this mutant (except at the seedling stage) had ruffled leaves with white stripes, sterile males, and poorly fertile female flowers. Genetic characteristics analysis revealed that the recessive gene controlled the virescent leaf. Two F populations mapped to the interval of 33.54-35.66 Mb on chromosome 3. In this interval, BSA-Seq, RNA-Seq, and cDNA sequence analyses revealed only one nonsynonymous mutation in the gene, which encoded the RNA exosome supercomplex subunit resurrection1 (RST1). was predicted to be the candidate gene controlling the virescent leaf, and the candidate gene may regulate chloroplast development by regulating . A transcriptome analysis showed that different factors caused the reduced chlorophyll and carotenoid content in the mutants. To our knowledge, this study is the first report of map-based cloning related to virescent leaf, male-sterile, and chloroplast RNA regulation in cucumber. The results could accelerate the study of the RNA exosome supercomplex for the dynamic regulation of chloroplast RNA.
叶绿体是光合作用的物质基础，温度和光照严重影响叶绿体发育，进而影响光合效率。本研究鉴定出一个自发的叶片黄化突变体SC311Y，其幼苗子叶和真叶均为黄色，并逐渐变绿。然而，温度和光照会影响其变绿过程。此外，该突变体（除苗期外）叶片卷曲且有白色条纹，雄性不育，雌花育性差。遗传特性分析表明，该黄化叶性状受隐性基因控制。两个F群体定位到3号染色体上33.54 - 35.66 Mb区间。在此区间，通过混合分组分析法测序（BSA-Seq）、RNA测序（RNA-Seq）和cDNA序列分析，发现只有一个基因发生非同义突变，该基因编码RNA外切体超复合体亚基resurrection1（RST1）。RST1被预测为控制黄化叶的候选基因，该候选基因可能通过调控 来调节叶绿体发育。转录组分析表明，不同因素导致突变体中叶绿素和类胡萝卜素含量降低。据我们所知，本研究是黄瓜中与黄化叶、雄性不育和叶绿体RNA调控相关的图位克隆的首次报道。这些结果可能会加速对RNA外切体超复合体动态调控叶绿体RNA的研究。
