Hematology Department, Hefei First People's Hospital, Hefei 23000, China.
Comput Intell Neurosci. 2022 Aug 23;2022:8741989. doi: 10.1155/2022/8741989. eCollection 2022.
In this paper, we have focused on the investigation of the expression level of the enhancer of zeste homolog 2 (EZH2) gene in bone marrow mononuclear cells of acute myeloid leukemia (AML) patients and analyze the relationship between EZH2 gene expression and EMI. The expression of EZH2mRNA in bone marrow mononuclear cells of 26 patients with incipient AML was detected by qRT-PCR, and the relationship between EZH2mRNA expression and clinical characteristics was analyzed. EZH2 mRNA expression was increased in 26 AML patients. EZH2 gene expression in male patients was significantly higher than that in female patients. The expression of EZH2 in the group with extramedullary infiltration (EMI) was significantly higher than that in the group without EMI. The patients were divided into different groups according to the chromosomal karyotype and prognosis. Statistical analysis showed that the expression level of the medium-risk group was significantly higher than that of the low-risk group, while there was no statistical difference in other groups ( > 0.05). The expression of EZH2 gene in AML patients was closely related to EMI, and the expression of EZH2 in AML cells was closely related to cell migration ability. EZH2 is expected to be one of the indicators of disease recurrence.
在本文中，我们专注于研究急性髓细胞白血病（AML）患者骨髓单个核细胞中增强子结合蛋白 2（EZH2）基因的表达水平，并分析 EZH2 基因表达与 EMI 之间的关系。通过 qRT-PCR 检测 26 例初发 AML 患者骨髓单个核细胞中 EZH2mRNA 的表达，并分析 EZH2mRNA 表达与临床特征的关系。结果发现，26 例 AML 患者中 EZH2mRNA 表达均增加。男性患者 EZH2 基因表达明显高于女性患者。有髓外浸润（EMI）组 EZH2 的表达明显高于无 EMI 组。根据染色体核型和预后将患者分为不同组。统计分析表明，中危组的表达水平明显高于低危组，而其他组之间无统计学差异（>0.05）。AML 患者 EZH2 基因的表达与 EMI 密切相关，AML 细胞中 EZH2 的表达与细胞迁移能力密切相关。EZH2 有望成为疾病复发的指标之一。
