Department of Rheumatology, Ankara City Hospital, Ankara, Turkey.
Department of Biochemistry, Ankara City Hospital, Ankara Yıldırım Beyazıt University, Ankara, Turkey.
Lab Med. 2023 Mar 7;54(2):166-172. doi: 10.1093/labmed/lmac084.
We aimed to investigate the plasma levels of tryptophan (Trp) and its metabolites in patients with primary Sjögren's syndrome (pSS).
The study included 34 pSS patients and 42 healthy individuals, and serum Trp and kynurenine (Kyn) concentrations were measured by liquid chromatography with tandem mass spectrometry. Trp degradation was predicted using the ratio of Kyn and Trp concentrations (Kyn/Trp).
In our study, the mean serum Trp concentration was found to be considerably lower in the pSS group than in the control group (P = .001). The levels of Kyn (P = .019) and the Kyn/Trp ratio (P < .001) were significantly higher in the pSS group than in the control group. The Kyn/Trp ratio was negatively correlated with C-reactive protein (r = -0.369, P = .032).
We found that Kyn pathway metabolism was altered in patients with pSS. This suggests that Trp metabolism may be closely linked to the disease pathogenesis of pSS.
本研究旨在探讨原发性干燥综合征（pSS）患者血浆色氨酸（Trp）及其代谢物的水平。
该研究纳入 34 例 pSS 患者和 42 名健康对照者，采用液相色谱-串联质谱法检测血清 Trp 和犬尿氨酸（Kyn）浓度。采用 Kyn 与 Trp 浓度比值（Kyn/Trp）预测 Trp 降解情况。
本研究中，pSS 组患者血清 Trp 浓度明显低于对照组（P=0.001）。pSS 组 Kyn（P=0.019）和 Kyn/Trp 比值（P<0.001）均显著高于对照组。Kyn/Trp 比值与 C 反应蛋白呈负相关（r=-0.369，P=0.032）。
本研究发现 pSS 患者的犬尿氨酸途径代谢发生改变。这提示 Trp 代谢可能与 pSS 的发病机制密切相关。
