Department of Periodontology, Faculty of Dentistry, School of Dentistry, Ankara University, 06500, Cankaya, Ankara, Turkey.
Department of Periodontology, Faculty of Dentistry, Medipol University, İstanbul, Turkey.
Clin Oral Investig. 2022 Sep;26(9):5721-5732. doi: 10.1007/s00784-022-04528-4. Epub 2022 May 19.
Kynurenine pathway (KP) is the primary way of degrading tryptophan (TRP) and generates several bioactive metabolites (such as kynurenine (KYN), kynurenic acid (KYNA), 3-hydroxykynurenine (3OHKYN)) to regulate biological processes that include host-microbiome signaling and immune cell response. This study is aimed to determine the relationship between periodontal inflammation and tryptophan-kynurenine metabolism and identify their association with periodontal clinical parameters.
Saliva and serum samples were collected from 20 stage III, grade B generalized periodontitis patients, and 20 periodontally healthy control individuals. Samples were analyzed for IL-6, KYN, TRP, KYN/TRP ratio, KYNA, 3OHKYN, picolinic acid (PA), and quinolinic acid (QA) by liquid chromatography-mass spectrometry. Clinical periodontal parameters (plaque index (PI), probing pocket depth (PPD), gingival recession (GR), clinical attachment loss (CAL), and bleeding on probing (BOP)) were recorded.
Clinical parameters were significantly higher in the periodontitis group (p < 0.001). Salivary IL-6, TRP, KYN, KYNA, PA, and QA levels were significantly higher and KYN/TRP ratio was significantly lower in periodontitis group than control group (p < 0.05). Serum KYN, KYN/TRP ratio and PA levels were significantly higher in periodontitis group than control group (p < 0.05). PPD, BOP, PI, and CAL had significantly positive correlations with salivary IL-6, TRP, PA, QA, and serum KYN and significantly negative correlations with salivary KYN/TRP ratio.
Our results suggest that periodontal inflammation plays a role in local and systemic tryptophan-kynurenine metabolism.
Due to their effects on the immune and inflammatory systems, kynurenines may be potential agents for diagnosis and treatment of periodontal diseases.
色氨酸-犬尿氨酸途径（KP）是降解色氨酸（TRP）的主要途径，产生几种生物活性代谢物（如犬尿氨酸（KYN）、犬尿氨酸酸（KYNA）、3-羟基犬尿氨酸（3OHKYN）），以调节包括宿主-微生物群信号和免疫细胞反应在内的生物过程。本研究旨在确定牙周炎与色氨酸-犬尿氨酸代谢的关系，并确定它们与牙周临床参数的关联。
收集 20 例 III 期 B 级广泛性牙周炎患者和 20 例牙周健康对照者的唾液和血清样本。通过液相色谱-质谱法分析 IL-6、KYN、TRP、KYN/TRP 比值、KYNA、3OHKYN、吡啶酸（PA）和喹啉酸（QA）。记录临床牙周参数（菌斑指数（PI）、探诊袋深度（PPD）、牙龈退缩（GR）、临床附着丧失（CAL）和探诊出血（BOP））。
牙周炎组的临床参数明显高于对照组（p<0.001）。牙周炎组唾液中 IL-6、TRP、KYN、KYNA、PA 和 QA 水平明显升高，KYN/TRP 比值明显降低（p<0.05）。牙周炎组血清 KYN、KYN/TRP 比值和 PA 水平明显高于对照组（p<0.05）。PPD、BOP、PI 和 CAL 与唾液中 IL-6、TRP、PA、QA 和血清 KYN 呈显著正相关，与唾液中 KYN/TRP 比值呈显著负相关。
我们的研究结果表明，牙周炎在局部和全身色氨酸-犬尿氨酸代谢中起作用。
由于犬尿氨酸对免疫和炎症系统的影响，犬尿氨酸可能是牙周病诊断和治疗的潜在药物。
