Bioconversion Technology Division, Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, Gujarat, 388120, India.
Gujarat Biotechnology University, Near Gujarat International Finance Tec-City, Gandhinagar, Gujarat, 382355, India.
World J Microbiol Biotechnol. 2022 Sep 2;38(11):212. doi: 10.1007/s11274-022-03399-x.
Rice straw decomposition is an attractive solution to open-field burning but the traditional method has slow kinetics and takes 60-90 days to obtain mature compost. In this study, we propose to boost up the decomposition process by addition of a novel microbial consortium rich in lignocellulolytic microbes. C: N ratio of the compost reached 11.69% and degradation efficiency of cellulose and hemicellulose was found to be 64 and 87% respectively within 25 days. Lignocellulolytic activity of the microbial consortium was confirmed by plate and activity assay. These parameters clearly indicated that a mature compost was obtained in 25 days. The 16S rRNA gene amplicon sequencing and functional analysis of predicted genes indicated amino acid and carbohydrate metabolism as the major metabolic pathway during composting. The tertiary level of functional analysis revealed the major metabolic pathways in the bacterial communities as pentose phosphate pathway, glycolysis and tricarboxylic acid cycle.
稻草分解是解决野外燃烧的一种有吸引力的方法，但传统方法的动力学较慢，需要 60-90 天才能获得成熟的堆肥。在这项研究中，我们建议通过添加富含木质纤维素分解微生物的新型微生物联合体来促进分解过程。堆肥的 C:N 比达到 11.69%，纤维素和半纤维素的降解效率分别在 25 天内达到 64%和 87%。通过平板和活性测定证实了微生物联合体的木质纤维素分解活性。这些参数清楚地表明，在 25 天内获得了成熟的堆肥。16S rRNA 基因扩增子测序和预测基因的功能分析表明，在堆肥过程中，氨基酸和碳水化合物代谢是主要的代谢途径。功能分析的第三级水平揭示了细菌群落中的主要代谢途径为戊糖磷酸途径、糖酵解和三羧酸循环。
