Shojaee Sahar, Ravash Rudabeh, Shiran Behrouz, Ebrahimie Esmaeil
Department of Plant Breeding and Biotechnology, Shahrekord University, Shahr-e Kord, Iran.
Department of Plant Breeding and Biotechnology Faculty of Agriculture, Shahrekord University, Shahr-e Kord, Iran.
J Genet Eng Biotechnol. 2022 Sep 2;20(1):127. doi: 10.1186/s43141-022-00395-4.
Wheat is the most important cereal. One of the environmental stresses is drought that harm the production of many cereals and every year due to low rainfall and frequent droughts, the need to produce plants resistant to this stress is felt. Therefore, identification and evaluation of the genes involved in the production of this resistance in plants are of great importance. By identifying these genes and changing their expression, it is possible to produce resistant plants that can tolerate dehydration and drought, with at least a qualitative and quantitative reduction in yield.
Based on the meta-analysis results obtained in this study, in resistant cultivars ~ 4% (2394/61290) of the probe IDs decreased and ~ 4.5% (2670/61290) increased expression, furthermore in susceptible cultivars ~ 7% (4183/61290) of probe IDs decreased and ~ 6% (3591/61290) increased expression (P value ≤ 0.05). List of up- and downregulated genes was revealed, among the expressed genes of transcription factors Myb3, ethylene-responsive 5a, MIKC-type MADS-box WM24B, and salinity inducible ERF4 in resistant cultivars and transcription factors WRKY15, MADS-box TaAGL8, WRKY39, and Myb in susceptible cultivars, they showed a significant increase in expression, these transcription factors are of great importance in drought stress. Among them, ethylene responsive 5a in resistant cultivars by 3 times and Myb in susceptible cultivars by 2.6 times have shown the highest expression change. Using Cytoscape Hub software, the Phosphoenolpyruvate carboxylase (PEPC) and lyase isocitrate (TaSAG7) genes, which have significantly different expressions in resistant and susceptible wheat cultivars. PEPC and TaSAG7 genes were upregulated in resistant wheat cultivars as well as down regulated in susceptible cultivars. Also, the qPCR results of selected genes were consistent with the outcomes of the meta-analysis.
All microarray data were collected from the NCBI Gene Expression Omnibus site. Libraries with drought-tolerant and susceptible cultivars for wheat were considered under the stress and control conditions from whole leaf tissue. By meta-analysis combined the purposeful results of multiple experiments, and found list of genes expressed in reverse between the two cultivars. These genes can distinguish between different susceptible and resistant wheat cultivars.
小麦是最重要的谷物。干旱是环境胁迫之一，它会损害许多谷物的产量，由于降雨少和干旱频繁，每年都需要培育抗这种胁迫的植物。因此，鉴定和评估植物中参与产生这种抗性的基因非常重要。通过鉴定这些基因并改变它们的表达，有可能培育出能够耐受脱水和干旱的抗性植物，至少在产量上有定性和定量的降低。
基于本研究获得的荟萃分析结果，在抗性品种中，约4%（2394/61290）的探针ID表达降低，约4.5%（2670/61290）的探针ID表达增加；此外，在感病品种中，约7%（4183/61290）的探针ID表达降低，约6%（3591/61290）的探针ID表达增加（P值≤0.05）。揭示了上调和下调基因列表，在抗性品种中，转录因子Myb3、乙烯响应因子5a、MIKC型MADS盒WM24B和盐诱导ERF4以及在感病品种中转录因子WRKY15、MADS盒TaAGL8、WRKY39和Myb的表达基因中，它们的表达显著增加，这些转录因子在干旱胁迫中非常重要。其中，抗性品种中的乙烯响应因子5a表达变化最高达3倍，感病品种中的Myb表达变化最高达2.6倍。使用Cytoscape Hub软件，磷酸烯醇丙酮酸羧化酶（PEPC）和异柠檬酸裂解酶（TaSAG7）基因在抗性和感病小麦品种中的表达有显著差异。PEPC和TaSAG7基因在抗性小麦品种中上调，在感病品种中下调。此外，所选基因的qPCR结果与荟萃分析结果一致。
所有微阵列数据均从NCBI基因表达综合数据库网站收集。在胁迫和对照条件下，考虑了来自全叶组织的耐旱和感病小麦品种文库。通过荟萃分析整合多个实验的有目的结果，发现了两个品种中反向表达的基因列表。这些基因可以区分不同的感病和抗性小麦品种。
