Røigaard-Petersen H, Jacobsen C, Iqbal Sheikh M
Am J Physiol. 1987 Jul;253(1 Pt 2):F15-20. doi: 10.1152/ajprenal.1987.253.1.F15.
The mechanism of renal transport of L-proline by luminal-membrane vesicles isolated from proximal convoluted tubules of rabbit kidney was studied. It was found that H+ gradient (extravesicular greater than intravesicular) can drive the transport of L-proline into the vesicles both in the presence and absence of Na+. The stimulation of L-proline uptake by a pH gradient was additive with that produced by Na+. Saturation kinetic experiments revealed that pH gradient, in addition to Na+, increased the maximal uptake of L-proline by twofold. This is the first demonstration of H+-L-proline cotransport across luminal membrane of rabbit kidney proximal convoluted tubule. The physiological importance of this system is briefly discussed.
对从兔肾近曲小管分离的管腔膜囊泡中L-脯氨酸的肾脏转运机制进行了研究。结果发现，无论有无Na+，H+梯度（囊泡外大于囊泡内）均可驱动L-脯氨酸转运进入囊泡。pH梯度对L-脯氨酸摄取的刺激作用与Na+产生的刺激作用具有加和性。饱和动力学实验表明，除Na+外，pH梯度使L-脯氨酸的最大摄取量增加了两倍。这是首次证明H+-L-脯氨酸共转运穿过兔肾近曲小管的管腔膜。简要讨论了该系统的生理重要性。
