质子梯度而非钠梯度是家兔小肠刷状缘膜囊泡中乳酸主动转运的驱动力。
A proton gradient, not a sodium gradient, is the driving force for active transport of lactate in rabbit intestinal brush-border membrane vesicles.
作者信息
Tiruppathi C, Balkovetz D F, Ganapathy V, Miyamoto Y, Leibach F H
机构信息
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
出版信息
Biochem J. 1988 Nov 15;256(1):219-23. doi: 10.1042/bj2560219.
Abstract
An inward-directed H+ gradient markedly stimulated lactate uptake in rabbit intestinal brush-border membrane vesicles, and uphill transport against a concentration gradient could be demonstrated under these conditions. Uptake of lactate was many-fold greater in the presence of a H+ gradient than in the presence of a Na+ gradient. Moreover, there was no evidence for uphill transport of lactate in the presence of a Na+ gradient. The H+-gradient-dependent stimulation of lactate uptake was not due to the effect of a H+-diffusion potential. The uptake process in the presence of a H+ gradient was saturable [Kt (concn. giving half-maximal transport) for lactate 12.7 +/- 4.5 mM] and was inhibited by many monocarboxylates. It is concluded that a H+ gradient, not a Na+ gradient, is the driving force for active transport of lactate in rabbit intestinal brush-border membrane vesicles.
摘要
内向的H⁺梯度显著刺激了兔肠刷状缘膜囊泡对乳酸的摄取，并且在这些条件下可以证明存在逆浓度梯度的上坡转运。在存在H⁺梯度的情况下，乳酸的摄取比存在Na⁺梯度时大许多倍。此外，没有证据表明在存在Na⁺梯度的情况下乳酸存在上坡转运。H⁺梯度依赖性的乳酸摄取刺激并非由于H⁺扩散电位的作用。在存在H⁺梯度的情况下，摄取过程是可饱和的（乳酸的Kt（产生半最大转运的浓度）为12.7±4.5 mM），并且受到许多单羧酸盐的抑制。得出的结论是，H⁺梯度而非Na⁺梯度是兔肠刷状缘膜囊泡中乳酸主动转运的驱动力。
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2
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Biochem J. 1980 Jan 15;186(1):169-76. doi: 10.1042/bj1860169.
3
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