Weiss Katie M, Kucko Sierra K, Mokhtari Sahar, Keenan Timothy J, Wren Anthony William
Inamori School of Engineering, Alfred University, Alfred, New York, USA.
J Biomed Mater Res B Appl Biomater. 2023 Feb;111(2):295-313. doi: 10.1002/jbm.b.35151. Epub 2022 Sep 2.
Hydroxyapatite (HA) powders were synthesized by the wet precipitation method in which two experimental compositions were synthesized (10 mol% Ag-HA and Cu-HA) where the CaNO content was partially substituted with AgNO and Cu(NO ) . X-ray diffraction (XRD) was employed to characterize changes to the HA structure as the dopants (Cu , Ag ) were incorporated into the materials structure. Energy-dispersive X-ray spectroscopy (EDS) determined confirmed the compositions and found that the Ca/P ratio was 1.63 for the control (HA) while Ag-HA and Cu-HA exhibited (X + Ca)/P ratios of 1.79 and 1.65, respectively. Antibacterial efficacies were evaluated against E. coli and S. aureus, as a function of surface area and incubation time. The more prominent antibacterial effects were observed with both Ag-HA and Cu-HA and the materials antibacterial influence was maintained with respect to time. Ion release studies of each HA composition (15, 30, and 45 days) showed that Cu-HA released significantly more Cu (36.1 ± 5.1 mg/L) than Ag (2.9 ± 1.2 mg/L) from Ag-HA. Analysis of each composition incubated in simulated body fluid (SBF) exhibited surface depositions that are likely calcium phosphate (CaP). Cytocompatibility testing in MC 3T3 Osteoblasts showed slight reductions in cell viability when tested using MTT assay, however cell adhesion studies were positive for each composition.
采用湿沉淀法合成了羟基磷灰石（HA）粉末，合成了两种实验成分（10 mol% Ag-HA和Cu-HA），其中CaNO含量部分被AgNO和Cu(NO)取代。采用X射线衍射（XRD）来表征随着掺杂剂（Cu、Ag）掺入材料结构中HA结构的变化。能量色散X射线光谱（EDS）确定了成分，发现对照组（HA）的Ca/P比为1.63，而Ag-HA和Cu-HA的（X + Ca）/P比分别为1.79和1.65。针对大肠杆菌和金黄色葡萄球菌评估了抗菌效果，作为表面积和孵育时间的函数。在Ag-HA和Cu-HA中均观察到更显著的抗菌效果，并且材料的抗菌影响随时间得以维持。对每种HA成分（15、30和45天）的离子释放研究表明，Cu-HA释放的Cu（36.1±5.1 mg/L）比Ag-HA释放的Ag（2.9±1.2 mg/L）显著更多。在模拟体液（SBF）中孵育的每种成分的分析显示出可能是磷酸钙（CaP）的表面沉积物。在MC 3T3成骨细胞中的细胞相容性测试表明，使用MTT法测试时细胞活力略有降低，然而每种成分的细胞粘附研究结果均为阳性。
