Chen W, Liu Y, Courtney H S, Bettenga M, Agrawal C M, Bumgardner J D, Ong J L
University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Biomaterials. 2006 Nov;27(32):5512-7. doi: 10.1016/j.biomaterials.2006.07.003. Epub 2006 Jul 26.
Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 degrees C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.
种植体植入后的细菌感染是一种显著增加的并发症。为了降低种植体相关感染的发生率，人们提出了几种生物材料表面处理方法。在本研究中，评估了共溅射含银（Ag）羟基磷灰石（HA）涂层的体外抗菌活性和体外细胞毒性。通过同时向Ag靶提供10 W功率和向HA靶提供300 W功率来实现沉积。沉积3小时后在400℃下进行4小时的热处理。使用X射线衍射、接触角测量和表面粗糙度来表征涂层表面。使用表皮葡萄球菌RP12菌株（ATCC 35984）和金黄色葡萄球菌考恩I菌株来评估Ag-HA涂层的抗菌活性，而使用人胚胎腭间充质细胞（一种成骨细胞前体细胞系）来评估涂层的体外细胞毒性。本研究中进行的X射线衍射分析表明，在共溅射的Ag-HA表面上有对应于Ag和HA的峰。与钛（Ti）表面相比，观察到HA和Ag-HA表面的接触角显著更低，而所有组在表面粗糙度上未观察到显著差异。体外细菌黏附研究表明，与钛（Ti）和HA表面相比，Ag-HA表面上的表皮葡萄球菌和金黄色葡萄球菌数量显著减少。此外，HA和Ag-HA表面之间在体外细胞毒性方面未观察到显著差异。总体而言，得出的结论是，通过将具有骨传导性的HA与抗菌Ag共溅射可以实现多功能表面的创建。
