Kraushaar J, Pfitzer P
Urol Int. 1978;33(4):205-12. doi: 10.1159/000280200.
Cytologic examinations of kidneys, which were frozen for preservation in a nitrogen atmosphere below 3 ata, show a considerable damage of the cells. This does not only occur by significant reduction in the amount of isolated cells, but also in the loss of the ability to form cell colonies. DNA measurements show, however, that after 3--4 days the DNA amounts in surviving cells are intact. In one of the cultures we even found DNA values, which stand for a DNA synthesis. Experiments with mechanical perfusion alone show that this procedure results in a severe damage of the cells, only about one-third of these cells being able to form colonies.
对在低于3个大气压的氮气环境中冷冻保存的肾脏进行细胞学检查，结果显示细胞受到了相当大的损伤。这不仅表现为分离细胞数量的显著减少，还表现为形成细胞集落能力的丧失。然而，DNA测量结果表明，在3至4天后，存活细胞中的DNA含量保持完整。在其中一种培养物中，我们甚至发现了代表DNA合成的DNA值。仅进行机械灌注的实验表明，该操作会导致细胞严重受损，只有约三分之一的细胞能够形成集落。
