Yang Riwei, Chen Zude, Liang Leqi, Ao Shan, Zhang Jinhu, Chang Zhenglin, Wang Zuomin, Zhou Yuhao, Duan Xiaolu, Deng Tuo
Department of Urology and Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Immunology. 2023 Feb;168(2):302-319. doi: 10.1111/imm.13557. Epub 2022 Aug 22.
Although recent studies have revealed the relationship between Fc Fragment of IgE Receptor Ig (FCER1G) and human tumours, there is still a lack of a more comprehensive pan-cancer analysis of FCER1G as an immune-related gene. In this study, we investigated the expression pattern and prognostic value of FCER1G based on multiple databases. Subsequently, we further explored the role of FCER1G in tumour proliferation and metastasis, as well as its genomic alterations and DNA methylation levels, we next assessed the association between FCER1G and the immune infiltrating cells of the tumour microenvironment in different cancers and verified it by immunohistochemical staining. The correlation between FCER1G and immune checkpoint genes expression and its predictive power in the immune checkpoint blockade treatment cohorts were used to evaluate the importance of FCER1G in immunotherapy. Enrichment analysis of FCER1G-associated partners was also performed. In addition, we substantiated the expression of FCER1G in specific cell types of different tumours using single-cell RNA sequencing data from different databases. Our research results showed that FCER1G is up-regulated in most tumour. Positive associations were found between FCER1G expression and tumour prognosis, proliferation, and metastasis, we also found that FCER1G is closely related to the tumour microenvironment and tumour immunity. Moreover, FCER1G-associated partners were enriched in pathways associated with neutrophils activation. Finally, we confirmed that FCER1G was mainly expressed in monocyte/macrophages of the tumour microenvironment. In conclusion, our findings provided a comprehensive understanding of FCER1G in oncogenesis and tumour immunology among various tumours and demonstrated its potential value in prognosis prediction and tumour immunotherapy.
尽管最近的研究揭示了免疫球蛋白E受体Ig（FCER1G）的Fc片段与人类肿瘤之间的关系，但作为一个免疫相关基因，对FCER1G仍缺乏更全面的泛癌分析。在本研究中，我们基于多个数据库研究了FCER1G的表达模式和预后价值。随后，我们进一步探讨了FCER1G在肿瘤增殖和转移中的作用，以及其基因组改变和DNA甲基化水平，接着我们评估了FCER1G与不同癌症中肿瘤微环境的免疫浸润细胞之间的关联，并通过免疫组织化学染色进行了验证。利用FCER1G与免疫检查点基因表达之间的相关性及其在免疫检查点阻断治疗队列中的预测能力来评估FCER1G在免疫治疗中的重要性。还对FCER1G相关伙伴进行了富集分析。此外，我们使用来自不同数据库的单细胞RNA测序数据证实了FCER1G在不同肿瘤的特定细胞类型中的表达。我们的研究结果表明，FCER1G在大多数肿瘤中上调。发现FCER1G表达与肿瘤预后、增殖和转移呈正相关，我们还发现FCER1G与肿瘤微环境和肿瘤免疫密切相关。此外，FCER1G相关伙伴在与中性粒细胞激活相关的途径中富集。最后，我们证实FCER1G主要在肿瘤微环境的单核细胞/巨噬细胞中表达。总之，我们的研究结果提供了对FCER1G在各种肿瘤的肿瘤发生和肿瘤免疫学中的全面理解，并证明了其在预后预测和肿瘤免疫治疗中的潜在价值。
