Department of Bioengineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, 70200 Karaman, Turkey.
Vocational School of Technical Sciences, Karamanoğlu Mehmetbey University, 70100 Karaman, Turkey; Scientific and Technological Research & Application Center, Karamanoğlu Mehmetbey University, 70100 Karaman, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5;284:121798. doi: 10.1016/j.saa.2022.121798. Epub 2022 Aug 28.
Fenamiphos (Fena), an organophosphorous pesticide, is widely used in agricultural soils to control nematodes and thrips. This nematicide is harmful to fish, birds and humans and, causes several diseases. Therefore, the determination of the nematicide is crucial. Fena has been generally detected by enzyme-based systems which require specific conditions. Herein, we integrated a xanthene moiety and a pyrimidine moiety to obtain an enzyme-free detection system for Fena and, a fluorescent sensor (N-(6-(diethylamino)-9-(pyrimidin-5-yl)-3H-xanthen-3-ylidene)-N-ethylethanaminium hexafluorophosphate(V)) (RosPm) was easily prepared. The colorimetric and spectroscopic properties of RosPm were investigated using the UV-vis and fluorescence spectroscopy. RosPm exhibited a high selectivity and sensitivity to Fena over all the metal ions, the anions and pesticides tested in acetonitrile (ACN)/water (HO) (v:v, 1:1) solution. RosPm showed a clear visual change from purple to light-purple resulting fluorescent quenching with Fena. This sensor could be preferred for detecting Fena in vegetable samples such as tomato, pepper, and cucumber, and visualizing Fena in living MFC-7 cells.
苯线磷（Fena）是一种有机磷农药，广泛用于农业土壤中防治线虫和蓟马。这种杀线虫剂对鱼类、鸟类和人类有害，并导致多种疾病。因此，对线虫剂的测定至关重要。Fena 通常通过基于酶的系统检测，该系统需要特定的条件。在此，我们整合了氧杂蒽部分和嘧啶部分，以获得用于 Fena 的无酶检测系统，并轻松制备了荧光传感器（N-（6-（二乙氨基）-9-（嘧啶-5-基）-3H-氧杂蒽-3-亚基）-N-乙基乙二胺六氟磷酸盐（V））（RosPm）。使用紫外可见光谱和荧光光谱研究了 RosPm 的比色和光谱性质。在乙腈（ACN）/水（HO）（v：v，1：1）溶液中，RosPm 对 Fena 表现出高度的选择性和灵敏度，超过了所有测试的金属离子、阴离子和农药。RosPm 与 Fena 发生反应后，颜色从紫色变为浅紫色，荧光猝灭。该传感器可用于检测番茄、辣椒和黄瓜等蔬菜样品中的 Fena，并可在活 MFC-7 细胞中可视化 Fena。
