Jiao Yuhong, Li Lu, Ge Jinlong, Tai Yanfang, Han Hui
School of Materials and Chemical Engineering, Bengbu University, Bengbu 233000, China.
Anhui Triumph Applied Materials Co., Ltd., Bengbu 233000, China.
Materials (Basel). 2024 Aug 7;17(16):3919. doi: 10.3390/ma17163919.
In this work, we demonstrated a novel, sensitive and effective fluorescent naringin (NRG) and naringenin (NRGe) detection method using polymethionine nanoparticles (PMNPs) as a fluorescent nanoprobe. The PMNPs were first synthesized by autopolymerization of methionine at 90 °C when trace copper ions existed. The as-prepared PMNPs were thoroughly characterized by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), gel permeation chromatograph (GPC), nuclear magnetic resonance spectroscopy (NMR), transient and steady-state fluorescence and UV-Vis absorption spectroscopy. The quenching mechanism was attributed to the inner filter effect (IFE). Moreover, the developed assay was used successfully to detect NRG and NRGe in real samples of citrus fruits, illustrating that this detection method has great potential application in the field of citrus fruits analysis.
在这项工作中，我们展示了一种新颖、灵敏且有效的荧光检测方法，该方法使用聚甲硫氨酸纳米颗粒（PMNPs）作为荧光纳米探针来检测柚皮苷（NRG）和柚皮素（NRGe）。当存在痕量铜离子时，甲硫氨酸在90℃下通过自聚合首先合成PMNPs。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）、凝胶渗透色谱（GPC）、核磁共振光谱（NMR）、瞬态和稳态荧光以及紫外-可见吸收光谱对所制备的PMNPs进行了全面表征。猝灭机制归因于内滤效应（IFE）。此外，所开发的检测方法成功用于检测柑橘类水果实际样品中的NRG和NRGe，表明该检测方法在柑橘类水果分析领域具有巨大的潜在应用价值。
