Dep. of Brain and Behavioral Sciences, University of Pavia, via Bassi 21, 27100 Pavia, Italy; Translational Neurovascular Research Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy.
Translational Neurovascular Research Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy.
Physiol Behav. 2022 Nov 1;256:113956. doi: 10.1016/j.physbeh.2022.113956. Epub 2022 Aug 31.
Migraine is a complex neurovascular disorder characterized by recurrent attacks of pain and other associated symptoms. Emotional-affective aspects are important components of pain, but so far they have been little explored in animal models of migraine. In this study, we aimed to explore the correlation between trigeminal hyperalgesia and affective status or behavioral components in a migraine-specific animal model. Male Sprague-Dawley rats were treated with nitroglycerin (10 mg/kg, i.p.) or its vehicle. Four hours later, anxiety, motor/exploratory behavior and grooming (a nociception index) were evaluated with the open field test. Rats were then exposed to formalin in the orofacial region to evaluate trigeminal hyperalgesia. The data analysis shows an inverse correlation between trigeminal hyperalgesia and motor or exploratory behavior, and a positive association with anxiety-like behavior or self-grooming. These findings further expand on the translational value of the migraine-specific model based on nitroglycerin administration and prompt additional parameters that can be investigated to explore migraine disease in its complexity.
偏头痛是一种复杂的神经血管性疾病，其特征是反复发作的疼痛和其他相关症状。情感方面是疼痛的重要组成部分，但迄今为止，它们在偏头痛的动物模型中研究甚少。在这项研究中，我们旨在探索偏头痛特异性动物模型中三叉神经痛觉过敏与情感状态或行为成分之间的相关性。雄性 Sprague-Dawley 大鼠接受硝酸甘油（10mg/kg，ip）或其载体处理。4 小时后，通过旷场试验评估焦虑、运动/探索行为和梳理（疼痛指数）。然后，将大鼠暴露于口腔区域的福尔马林中以评估三叉神经痛觉过敏。数据分析显示，三叉神经痛觉过敏与运动或探索行为呈负相关，与焦虑样行为或自我梳理呈正相关。这些发现进一步扩展了基于硝酸甘油给药的偏头痛特异性模型的转化价值，并提示可以研究其他参数来探索偏头痛疾病的复杂性。
