基于临床应用和动物行为分析的白芍（一种传统中药）对偏头痛的影响。
Effect of Paeonia lactiflora, a traditional Chinese herb, on migraines based on clinical application and animal behavior analyses.
机构信息
Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan.
Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Department of Chinese Medicine, Show Chwan Memorial Hospital, Changhua, 50008, Taiwan.
出版信息
Biomed Pharmacother. 2019 Oct;118:109276. doi: 10.1016/j.biopha.2019.109276. Epub 2019 Aug 1.
BACKGROUND
Paeonia lactiflora (PL) was widely used for pain relief, but its effects on migraine headaches remain unclear.
PURPOSE
The aim of the present study was to investigate the effects of PL on migraine headaches.
METHODS
First, we found that PL was frequently used in Taiwan for headache treatment based on data from Taiwan's National Health Insurance Research Database. Migraine was induced through the intraperitoneal injection (i.p.) of nitroglycerin (NTG, 10 mg/kg) in rats. Pretreatment with PL was administered orally 30 min prior to the NTG i.p. Migraine headache behavior was observed by video-recordings. Finally, the rats were sacrificed and brain was removed for immunohistochemistry staining analysis.
RESULTS
The frequency and total time spent rearing up and sniffing in exploratory behavior, and walking in locomotor behavior, were reduced in the NTG group compared with the control group (all p < 0.001). This reduction could be ameliorated by pretreatment with PL 1.0 g/kg (all p < 0.05). Total time spent in the light chamber was lower in the NTG group compared with the control group (p < 0.05); this could be ameliorated by pretreatment with 1.0 g/kg PL (p < 0.05). The rats in the NTG group spent longer time on the smooth surface than those in the control group (p < 0.001); this could be shortened by pretreatment with 0.5 and 1.0 g/kg PL (both p < 0.01). The traveling distance of rats in the NTG group was shorter than in the control group (p < 0.001); rats given 1.0 g/kg PL had a longer traveling distance than those in the NTG group (p < 0.01). Both c-fos and CGRP immunoreactive cells increased in the TNC in the NTG group compared with that of the control group (both p < 0.001); this increased could be reduced by pretreatment with PL 0.5 and 1.0 g/kg (both p < 0.05).
CONCLUSION
Pretreatment with PL ameliorated migraine headache behaviors in the NTG-induced migraine rat model, suggesting pretreatment with PL is beneficial for migraine headache treatment. This effect of PL is related to the decrease of c-fos and CGRP in the TNC. However, still there are too many methodological limitations which need to be overcome in further experiments to support the data.
背景
白芍（PL）被广泛用于缓解疼痛，但它对偏头痛的影响尚不清楚。
目的
本研究旨在探讨白芍对偏头痛的影响。
方法
首先，我们根据台湾全民健康保险研究数据库的数据发现，白芍在台湾常用于头痛治疗。通过腹腔注射（i.p.）硝化甘油（NTG，10mg/kg）在大鼠中诱导偏头痛。在 NTG i.p. 之前 30 分钟给予 PL 预处理，通过视频记录观察偏头痛头痛行为。最后，处死大鼠并取出大脑进行免疫组织化学染色分析。
结果
与对照组相比，NTG 组大鼠在探索行为中的后肢站立和嗅探频率和总时间以及在运动行为中的行走次数减少（均 p<0.001）。用 1.0g/kg PL 预处理可以减轻这种减少（均 p<0.05）。与对照组相比，NTG 组大鼠在亮箱中的总时间较低（p<0.05）；用 1.0g/kg PL 预处理可以减轻这种情况（p<0.05）。与对照组相比，NTG 组大鼠在光滑表面上花费的时间更长（p<0.001）；用 0.5 和 1.0g/kg PL 预处理可以缩短此时间（均 p<0.01）。NTG 组大鼠的行驶距离短于对照组（p<0.001）；用 1.0g/kg PL 预处理的大鼠行驶距离长于 NTG 组（p<0.01）。与对照组相比，NTG 组大鼠中 TNC 中的 c-fos 和 CGRP 免疫反应细胞增加（均 p<0.001）；用 0.5 和 1.0g/kg PL 预处理可以减少这种增加（均 p<0.05）。
结论
PL 预处理可改善 NTG 诱导的偏头痛大鼠模型中的偏头痛头痛行为，提示 PL 预处理有益于偏头痛头痛治疗。PL 的这种作用与 TNC 中 c-fos 和 CGRP 的减少有关。然而，在进一步的实验中仍然存在许多方法学限制，需要克服这些限制才能支持这些数据。
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