Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland; Department of Infectious Diseases, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
J Immunol Methods. 2022 Nov;510:113345. doi: 10.1016/j.jim.2022.113345. Epub 2022 Aug 30.
Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8-3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen.
定量抗体反应的测量在评估感染和疫苗接种的免疫反应方面变得越来越重要。在这项研究中，我们描述了一种利用电化学发光平台的定量、多重血清学检测方法的验证，该方法可测量针对 SARS-CoV-2 的受体结合域 (RBD)、刺突 S1 和 S2 亚基以及核衣壳抗原的 IgG。该检测方法在检测 SARS-CoV-2 既往感染方面的灵敏度为 73%至 91%，特异性为 90%至 96%，具体取决于抗原靶标和感染后时间，并且该检测方法与市售检测方法高度相关。每个抗原的板内变异系数范围为 3.8-3.9%，板间变异系数范围为 11-13%。
