College of Medicine, Ajman University, Ajman, United Arab Emirates; Center of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates; Department of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.
Division of Biochemistry, Department of Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland.
Prog Neuropsychopharmacol Biol Psychiatry. 2023 Jan 10;120:110625. doi: 10.1016/j.pnpbp.2022.110625. Epub 2022 Aug 30.
Binge eating episodes are persistent and are essential features of numerous eating disorders (EDs). Susceptibility to EDs is largely presumed to be associated with early life stress. In fact, converging evidence from preclinical animal studies have implicated stress as a driver of binge eating. Still, literature examination indicates that vulnerability to EDs may depend on factors such as severity, time, and the type of stressor. Therefore, we aimed at exploring the link between chronic psychosocial stress and 'binge-like' sucrose intake in adolescent mice. To this aim, intruders' experimental mice were exposed to the chronic subordinate colony (CSC) housing, in the presence of a resident aggressive mouse for 2 weeks. At the end of the stress period, mice were tested for anxiety-like behavior then assessed for 'binge-like' intake of sucrose using a long-term drinking in the dark (DID) method that successfully replicates binge eating in humans. As expected, and compared to single housed colony controls (SHC), CSC exposure elicited an anxiogenic-like response in the open field (OF) and elevated-plus maze (EPM) tests and reduced weight gain. Most importantly, we report here for the first time, that mice exposed to chronic psychosocial stress displayed a 'binge-like' consumption of sucrose. However, neither quinine (bitter) nor saccharin (sweet) intakes were affected by CSC exposure. Finally, using Pearson's correlation, results showed a strong correlation between anxiety-like behavior parameters and sucrose intake. Overall these findings support the validity of our chronic psychosocial stress to model binge EDs and establish the long-term consequences of stress on 'binge-like' eating in male mice. These data suggest that chronic psychosocial stress is a risk factor for developing anxiety-associated EDs.
狂食发作是持久的，并且是许多饮食失调（ED）的基本特征。ED 的易感性很大程度上被认为与早期生活压力有关。事实上，来自临床前动物研究的综合证据表明，压力是暴食的驱动因素。尽管如此，文献研究表明，对 ED 的易感性可能取决于严重程度、时间和压力源的类型等因素。因此，我们旨在探讨慢性心理社会压力与青春期小鼠“暴食样”蔗糖摄入之间的联系。为此，入侵者实验小鼠被暴露于慢性下属群体（CSC）住房中，同时存在一只攻击性常驻老鼠 2 周。在应激期结束时，对小鼠进行焦虑样行为测试，然后使用长期黑暗饮（DID）方法评估“暴食样”蔗糖摄入，该方法成功复制了人类的暴食行为。正如预期的那样，与单独饲养的群体对照（SHC）相比，CSC 暴露在开阔场（OF）和高架十字迷宫（EPM）测试中引起了焦虑样反应，并降低了体重增加。最重要的是，我们首次在这里报告，暴露于慢性心理社会压力的小鼠表现出“暴食样”蔗糖消耗。然而，苦味奎宁（苦）或甜蜜素（甜）的摄入量不受 CSC 暴露的影响。最后，使用 Pearson 相关分析，结果表明焦虑样行为参数与蔗糖摄入量之间存在很强的相关性。总体而言，这些发现支持了我们使用慢性心理社会压力来模拟暴食 ED 的有效性，并确定了应激对雄性小鼠“暴食样”进食的长期影响。这些数据表明，慢性心理社会压力是发展与焦虑相关的 ED 的危险因素。
