Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Fujian Institute of Tobacco Agricultural Sciences, Fuzhou 350003, China.
Genomics. 2022 Sep;114(5):110471. doi: 10.1016/j.ygeno.2022.110471. Epub 2022 Aug 31.
Ralstonia solanacearum severely damages the growth of tobacco (Nicotiana tabacum L.) and causes great economic losses in tobacco production. To investigate the root metabolism and transcriptional characteristics of tobacco bacterial wilt susceptible variety Cuibi-1 (CB-1) and resistant new line KCB-1 (derived from an ethyl methanesulfonate (EMS) mutant of CB-1) after infestation with R. solanacearum, root metabolism and transcriptional characteristics were investigated using RNA-Seq and liquid chromatography-mass spectrometry (LC-MS). Differences in resistance between KCB-1 and CB-1 were observed in several aspects: (1) The phenylpropanoid pathway was the main pathway of resistance to bacterial wilt in KCB-1 compared with CB-1. (2) KCB-1 had more differential metabolic markers of disease resistance than CB-1 after infection with R. solanacearum. Among them, the differential coumarin-like metabolites that affect quorum sensing (QS) and biofilm formation of R. solanacearum differ in KCB-1 and CB-1. (3) KCB-1 inhibited production of the R. solanacearum metabolite putrescine, and the level of putrescine in tobacco was positively correlated with susceptibility. (4) Compared with CB-1, the metabolites of KCB-1 had less differential nitrogen sources during the infestation of R. solanacearum, which was detrimental to the growth and reproduction of R. solanacearum. (5) Both indole-3-acetic acid (IAA) and abscisic acid (ABA) in CB-1 and KCB-1 were involved in the response to R. solanacearum infestation, but the levels of IAA and ABA in KCB-1 were greater than in CB-1 at 24 h post inoculation (hpi). In conclusion, R. solanacearum caused reprogramming of both root metabolism and transcription in KCB-1 and CB-1, and the transcriptional and metabolic characteristics of resistant tobacco were more unfavorable to R. solanacearum.
青枯雷尔氏菌严重损害烟草（Nicotiana tabacum L.）的生长，给烟草生产造成巨大经济损失。为了研究感病品种翠碧 1 （CB-1）和抗青枯雷尔氏菌新株系 KCB-1（源自 CB-1 的乙基甲磺酸酯（EMS）突变体）在受到青枯雷尔氏菌侵染后的根系代谢和转录特征，我们使用 RNA-Seq 和液相色谱-质谱（LC-MS）对根系代谢和转录特征进行了研究。KCB-1 和 CB-1 在几个方面表现出抗性差异：（1）与 CB-1 相比，苯丙烷途径是 KCB-1 抗青枯病的主要途径。（2）与 CB-1 相比，KCB-1 在感染青枯雷尔氏菌后具有更多的抗病差异代谢标志物。其中，影响青枯雷尔氏菌群体感应（QS）和生物膜形成的差异香豆素样代谢物在 KCB-1 和 CB-1 中不同。（3）KCB-1 抑制青枯雷尔氏菌代谢产物腐胺的产生，而烟草中的腐胺水平与感病性呈正相关。（4）与 CB-1 相比，KCB-1 在感染青枯雷尔氏菌时，其根系代谢物的氮源差异较小，这不利于青枯雷尔氏菌的生长和繁殖。（5）CB-1 和 KCB-1 中的吲哚-3-乙酸（IAA）和脱落酸（ABA）均参与了对青枯雷尔氏菌侵染的响应，但在接种后 24 小时（hpi），KCB-1 中的 IAA 和 ABA 水平高于 CB-1。总之，青枯雷尔氏菌引起 KCB-1 和 CB-1 的根系代谢和转录重新编程，而抗病烟草的转录和代谢特征对青枯雷尔氏菌更为不利。
