Wang Yao, Yan Min, Wang Anbin, Ma Xingjun, Tian Weiqiang, Liu Ying, Zhu Liquan, Ding Wei, Li Shili
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, China.
Yibin Tobacco Company of Sichuan Province, Yibin, China.
Front Plant Sci. 2025 Jun 5;16:1566215. doi: 10.3389/fpls.2025.1566215. eCollection 2025.
Plants will display typical dehydration and wilting symptoms after infection. Many studies have shown that abscisic acid (ABA) has been implicated in wilting, but the role of ABA after infection remains largely unexplored. The plant water potential and endogenous ABA content of tobacco plants were investigated after infection, and we assessed the preliminary mechanisms and control effect of exogenous ABA on tobacco bacterial wilt. Here we show that can reduce leaf water content (LWC) and leaf water potential (Ψ) and promote the accumulation of ABA on leaves. Notably, foliar spraying 0.78 mg/L ABA could alleviate the wilting by increasing Ψ and decreasing the stomatal size, stomatal conductance (Gs), and transpiration rate (Tr). Furthermore, 0.78 mg/L ABA application promoted plant growth, reduced the colonization of , increased the activities of defense enzymes, upregulated the expression of JA/ET-related and ROS-related genes, and suppressed the expression of SA-related gene. Moreover, 0.78 mg/L ABA could reduce the incidence of tobacco bacterial wilt, with the control efficiency reaching up to 54.94% at 11 dpi, significantly higher than that of benzothiazole (BTH) with 19.33%. Our findings provided a new result for exogenous ABA controlling tobacco bacterial wilt by reducing water loss and enhancing plant resistance.
感染后植物会表现出典型的脱水和萎蔫症状。许多研究表明脱落酸（ABA）与萎蔫有关，但感染后ABA的作用在很大程度上仍未得到探索。研究了感染后烟草植株的植物水势和内源ABA含量，并评估了外源ABA对烟草青枯病的初步作用机制和防治效果。在此我们表明，（原文此处“that”后内容缺失）可降低叶片含水量（LWC）和叶片水势（Ψ），并促进叶片上ABA的积累。值得注意的是，叶面喷施0.78 mg/L ABA可通过提高Ψ并减小气孔大小、气孔导度（Gs）和蒸腾速率（Tr）来缓解萎蔫。此外，施用0.78 mg/L ABA可促进植株生长，减少（原文此处“of”后内容缺失）的定殖，增加防御酶的活性，上调JA/ET相关基因和ROS相关基因的表达，并抑制SA相关基因的表达。此外，0.78 mg/L ABA可降低烟草青枯病的发病率，在接种后11天防治效率高达54.94%，显著高于苯并噻二唑（BTH）的19.33%。我们的研究结果为外源ABA通过减少水分流失和增强植物抗性来防治烟草青枯病提供了新的成果。
