Sakakibara R, Tominaga N, Sakai A, Ishiguro M
Anal Biochem. 1987 Apr;162(1):150-5. doi: 10.1016/0003-2697(87)90020-0.
An efficient method of separating proteins by electrophoresis with agarose gels containing sodium dodecyl sulfate is described. The separated proteins were then used to make antibodies. The separation of proteins in the molecular weight range 25K to 94K on 4 to 5% agarose gels showed good resolution which was comparable to that obtained by electrophoresis with polyacrylamide gels containing sodium dodecyl sulfate. The subunits of human chorionic gonadotropin were separated by the preparative agarose gel electrophoresis and the gel bands containing the subunits were used directly to raise antibodies against these proteins. The degree of solubilization of the proteins from the gel slices was found to be complete.
描述了一种用含十二烷基硫酸钠的琼脂糖凝胶通过电泳分离蛋白质的有效方法。然后将分离出的蛋白质用于制备抗体。在4%至5%的琼脂糖凝胶上对分子量范围为25K至94K的蛋白质进行分离，显示出良好的分辨率，这与用含十二烷基硫酸钠的聚丙烯酰胺凝胶电泳所获得的分辨率相当。通过制备性琼脂糖凝胶电泳分离出人绒毛膜促性腺激素的亚基，含有这些亚基的凝胶条带直接用于制备针对这些蛋白质的抗体。发现从凝胶切片中溶解蛋白质的程度是完全的。
