Cashaw J L, Geraghty C A, McLaughlin B R, Davis V E
Anal Biochem. 1987 Apr;162(1):274-82. doi: 10.1016/0003-2697(87)90037-6.
A sensitive and selective method for detection of tetrahydropapaveroline (THP) in rat brain has been developed. The procedure employs a multiple-stage separation scheme that selectively isolates THP from rat brain tissue and utilizes the sensitivity and resolution of reversed-phase high-performance liquid chromatography with electrochemical detection to provide an analysis with high specificity for THP. The mean (+/- SD) recovery of THP from rat brain homogenates, fortified at levels ranging from 0.25 to 3.0 pmol per whole brain, was 43.4 +/- 3.5%. The concentration of THP in brains of rats pretreated with L-dopa was 0.44 +/- 0.14 (SD) pmol per gram. The limit of detection of THP was approximately 0.1 pmol (0.03 ng) per gram brain.
已开发出一种灵敏且具选择性的方法用于检测大鼠脑中的四氢罂粟碱（THP）。该方法采用多阶段分离方案，能从大鼠脑组织中选择性分离出THP，并利用反相高效液相色谱结合电化学检测的灵敏度和分辨率，对THP进行高特异性分析。从全脑添加量为0.25至3.0 pmol的大鼠脑匀浆中回收THP的平均（±标准差）回收率为43.4±3.5%。用左旋多巴预处理的大鼠脑中THP的浓度为每克0.44±0.14（标准差）pmol。THP的检测限约为每克脑0.1 pmol（0.03 ng）。
