Institute of Public Health and Clinical Nutrition, School of Medicine, Faculty of Health Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland.
Food Chemistry and Food Development Unit, Department of Biochemistry, University of Turku, Itäinen Pitkäkatu 4, 20014, Turku, Finland.
Sci Rep. 2022 Sep 2;12(1):15018. doi: 10.1038/s41598-022-19327-w.
The essential role of gut microbiota in health and disease is well recognized, but the biochemical details that underlie the beneficial impact remain largely undefined. To maintain its stability, microbiota participates in an interactive host-microbiota metabolic signaling, impacting metabolic phenotypes of the host. Dysbiosis of microbiota results in alteration of certain microbial and host metabolites. Identifying these markers could enhance early detection of certain diseases. We report LC-MS based non-targeted metabolic profiling that demonstrates a large effect of gut microbiota on mammalian tissue metabolites. It was hypothesized that gut microbiota influences the overall biochemistry of host metabolome and this effect is tissue-specific. Thirteen different tissues from germ-free (GF) and conventionally-raised (MPF) C57BL/6NTac mice were selected and their metabolic differences were analyzed. Our study demonstrated a large effect of microbiota on mammalian biochemistry at different tissues and resulted in statistically-significant modulation of metabolites from multiple metabolic pathways (p ≤ 0.05). Hundreds of molecular features were detected exclusively in one mouse group, with the majority of these being unique to specific tissue. A vast metabolic response of host to metabolites generated by the microbiota was observed, suggesting gut microbiota has a direct impact on host metabolism.
肠道微生物群在健康和疾病中的重要作用已得到充分认识，但构成其有益影响的生化细节在很大程度上仍未得到明确。为了保持其稳定性，微生物群参与宿主-微生物群代谢信号的相互作用，影响宿主的代谢表型。微生物群的失调会导致某些微生物和宿主代谢物的改变。鉴定这些标志物可以增强对某些疾病的早期检测。我们报告了基于 LC-MS 的非靶向代谢组学分析，证明了肠道微生物群对哺乳动物组织代谢物有很大的影响。据推测，肠道微生物群影响宿主代谢组的整体生物化学，这种影响具有组织特异性。从无菌（GF）和常规饲养（MPF）C57BL/6NTac 小鼠中选择了 13 种不同的组织，并分析了它们的代谢差异。我们的研究表明，微生物群对不同组织中哺乳动物生物化学有很大的影响，并导致来自多个代谢途径的代谢物发生统计学上显著的调节（p≤0.05）。在一个小鼠组中检测到数百个分子特征，其中大多数是特定组织所特有的。宿主对微生物群产生的代谢物的广泛代谢反应表明，肠道微生物群对宿主代谢有直接影响。
