School of Life Sciences, Shanghai University, Shanghai, 200444, People's Republic of China.
School of Medicine, Shanghai University, Shanghai, 200444, People's Republic of China.
Mikrochim Acta. 2022 Sep 2;189(10):367. doi: 10.1007/s00604-022-05465-5.
A self-assembled nanozyme of iron porphyrin mediated supramolecular modified gold nanoparticles (FpA) was fabricated to determine nitrated alpha-synuclein as the Tyr 39 residue (nT39 α-Syn) of a potential biomarker for early diagnosis of Parkinson's disease (PD). Mechanically, localized surface plasmon resonance (LSPR) and the mass effect caused by catalytic deposition of the nanozyme contributed to a cascade signal amplification strategy. The sensor allowed a signal amplification and selective nT39 α-Syn bioanalysis with a 1.34-fold enhancement by cascade amplified SPR signal and double specific recognition. The detection limit was 1.78 ng/mL in the detection range of 7-240 ng/mL. Benefiting from the excellent immunosensor, this method can distinguish healthy people and PD patients using actual samples. Overall, this strategy provides a nanozyme-based biosensing platform for the early diagnosis of PD and can be applied to detect other protein biomarkers, such as PD-L1.
一种由铁卟啉介导的超分子修饰的金纳米粒子（FpA）自组装纳米酶被制备用于检测硝化的α-突触核蛋白作为潜在的帕金森病（PD）早期诊断生物标志物的 Tyr 39 残基（nT39α-Syn）。从机制上讲，纳米酶的局部表面等离子体共振（LSPR）和催化沉积引起的质量效应促成了级联信号放大策略。该传感器通过级联放大的 SPR 信号和双重特异性识别，允许信号放大和选择性的 nT39α-Syn 生物分析，信号增强 1.34 倍。在 7-240ng/mL 的检测范围内，检测限为 1.78ng/mL。得益于出色的免疫传感器，该方法可以使用实际样本区分健康人和 PD 患者。总的来说，该策略为 PD 的早期诊断提供了基于纳米酶的生物传感平台，并且可以应用于检测其他蛋白质生物标志物，如 PD-L1。
