Faculty of Health Sciences, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Stem Cell Institue, Ankara University, Ankara, Turkey.
Phys Chem Chem Phys. 2024 Feb 7;26(6):5106-5114. doi: 10.1039/d3cp04527g.
An innovative biosensing fabrication strategy has been demonstrated for the first time using a quartz tuning fork (QTF) to develop a practical immunosensor for sensitive, selective and practical analysis of alpha synuclein protein (SYN alpha), a potential biomarker of Parkinson's disease. Functionalization of gold-coated QTFs was carried out in 2 steps by forming a self-assembled monolayer with 4-aminothiophenol (4-ATP) and conjugation of gold nanoparticles (AuNPs). The selective determination range for SYN alpha of the developed biosensor system is 1-500 ng mL in accordance with the resonance frequency shifts associated with a limit of detection of 0.098 ng mL. The changes in surface morphology and elemental composition were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDX). The remarkable point of the study is that this QTF based mass sensitive biosensor system can capture the SYN alpha target protein in cerebrospinal fluid (CSF) samples with recoveries ranging from 92% to 104%.
首次展示了一种创新的生物传感制造策略，该策略使用石英音叉（QTF）开发了一种实用的免疫传感器，用于灵敏、选择性和实用地分析α突触核蛋白（SYN alpha），这是帕金森病的潜在生物标志物。金涂覆 QTF 的功能化分两步进行，先用 4-巯基苯胺（4-ATP）形成自组装单层，然后再连接金纳米颗粒（AuNPs）。根据与检测限 0.098 ng mL 相关的共振频率偏移，所开发的生物传感器系统对 SYN alpha 的选择性测定范围为 1-500 ng mL。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和能谱（EDX）评估了表面形貌和元素组成的变化。这项研究的显著之处在于，这种基于 QTF 的质量敏感生物传感器系统可以从脑脊液（CSF）样本中捕获 SYN alpha 靶蛋白，回收率范围为 92%至 104%。
