Ma Huiling, Yang Longbing, Tian Zhuqing, Zhu Lijuan, Peng Jian, Fu Ping, Xiu JiangFan, Guo Guo
The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, China.
Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Sanquan College of Xinxiang Medical University, Xinxiang, 453003, China.
Int Microbiol. 2023 Jan;26(1):81-90. doi: 10.1007/s10123-022-00274-5. Epub 2022 Sep 3.
There is a need for new anti-Candida albicans (C. albicans) drugs owing to the emergence of drug resistance in recent years. AMP-17, an antimicrobial peptide from Musca domestica (M. domestica), is known to be an effective inhibitor of many fungal pathogens, including C. albicans. In this study, we investigated the potential mechanism underlying the anti-C. albicans effects of AMP-17 using flow cytometry, transmission electron microscopy, fluorescent probes, fluorescence microplate reader, and confocal laser microscopy. Transmission electron microscopy showed that, following AMP-17 treatment, the shape of C. albicans cells became irregular, and vacuoles could be seen in the cytoplasm. Furthermore, AMP-17 treatment resulted in an increase in reactive oxygen species (ROS) levels, depolarization of the mitochondrial membrane potential (MMP), and changes in the cell cycle, leading to the apoptosis and necrosis, which ultimately contributed to the death of C. albicans cells.
由于近年来耐药性的出现，需要新型抗白色念珠菌药物。家蝇抗菌肽AMP-17是已知的对包括白色念珠菌在内的许多真菌病原体有效的抑制剂。在本研究中，我们使用流式细胞术、透射电子显微镜、荧光探针、荧光酶标仪和共聚焦激光显微镜研究了AMP-17抗白色念珠菌作用的潜在机制。透射电子显微镜显示，AMP-17处理后，白色念珠菌细胞形状变得不规则，细胞质中可见液泡。此外，AMP-17处理导致活性氧（ROS）水平升高、线粒体膜电位（MMP）去极化以及细胞周期变化，导致凋亡和坏死，最终导致白色念珠菌细胞死亡。
