Unitat Mixta d'investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Catalonia, Spain.
IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Catalonia, Spain.
Vet Res. 2022 Sep 2;53(1):67. doi: 10.1186/s13567-022-01084-3.
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10 TCID/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.
中东呼吸综合征冠状病毒（MERS-CoV）对公共卫生构成严重威胁。在这里，我们使用气液界面培养系统建立了体外羊驼气管外植体（ATE）模型，以深入了解骆驼下呼吸道中的 MERS-CoV 感染。ATE 可被 MERS-CoV 感染，建立有效感染所需的最低病毒剂量为 10TCID/mL。IFN 和抗病毒 ISG 在 ATE 培养物中未因 MERS-CoV 感染而被诱导，这强烈表明体内观察到的 ISG 表达更可能是骆驼鼻黏膜中 IFN 诱导的结果。
