Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Int J Biol Macromol. 2022 Oct 31;219:1155-1162. doi: 10.1016/j.ijbiomac.2022.08.184. Epub 2022 Aug 31.
Bacillus velezensis P1 inhibits various phytopathogenic fungi. Its exopolymeric substance (EPS) was active against Colletotrichum sp. at a minimum inhibitory concentration (MIC) of 6.25 mg/ml. EPS was added to chitosan from squid pen to produce an antifungal film coating. The addition of EPS at its MIC had no effect on the color of the chitosan film. Two components showed good compatibility and the tensile strength of the film was higher than that of neat chitosan film. Mangoes were treated with six different coatings, including the two films. Fruits coated with the films showed the lowest weight loss and prolonged shelf life of mangoes to three times the shelf life of controls (water and 2 % citric acid, w/v) and completely inhibited fungal infection. Film coatings of chitosan, alone or combined with the EPS from B. velezensis P1, showed potential to prolong the shelf life of ripe mangoes and decrease postharvest losses.
贝莱斯芽孢杆菌 P1 可抑制多种植物病原菌。其胞外聚合物(EPS)在最低抑菌浓度(MIC)为 6.25mg/ml 时对炭疽菌属具有活性。将 EPS 添加到鱿鱼墨制成的壳聚糖中,以生产出一种具有抗真菌作用的涂膜。在 MIC 下添加 EPS 对壳聚糖膜的颜色没有影响。两种成分具有良好的相容性,并且膜的拉伸强度高于纯壳聚糖膜。将六种不同的涂层(包括这两种膜)应用于芒果。用膜处理过的芒果的失重率最低,货架期延长了三倍,是对照(水和 2%柠檬酸,w/v)的三倍,并且完全抑制了真菌感染。壳聚糖单独或与贝莱斯芽孢杆菌 P1 的 EPS 联合使用的涂膜具有延长成熟芒果货架期和减少采后损失的潜力。