Portnoy J, Chapman J, Burge H, Muilenberg M, Solomon W
Ann Allergy. 1987 Jul;59(1):39-43.
Comparable degrees of skin reactivity were observed towards spore and mycelium extracts from two isolates of Epicoccum and to one preparation of Alternaria in 35 rural and 120 university patients. The best experimental extracts detected Epicoccum sensitivity in 70% of the group tested while the commercial extract detected sensitivity in only 6%. Skin reaction correlations were greatest within isolates (eg, spore-A/mycelium-A), then for specific fungus parts (eg, spore-A/spore-B), then between isolates and parts (spore-A/mycelium-B). High correlations were found between individual IgG and IgE ELISA values for all antigens using serum from Epicoccum skin-reactive patients. ELISA inhibition results suggested that significant cross-reactivity exists between Epicoccum and Alternaria antigens recognized by IgG but not by IgE. ELISA inhibition cross-reaction patterns among Epicoccum antigens were comparable to skin reactions while IgG patterns showed little variability. Further characterization of spore/mycelium and interstrain recognition patterns among different immunoglobulin isotypes will be necessary before complete standardization of extracts from different parts of fungi will be possible. The use of spore material for skin testing and treatment of Epicoccum sensitivity appears to be both premature and unnecessary at this time.
在35名农村患者和120名大学患者中，观察到对两种附球菌分离株的孢子和菌丝体提取物以及一种链格孢制剂的皮肤反应程度相当。最佳实验提取物在70%的受试组中检测到附球菌敏感性，而商业提取物仅在6%的受试组中检测到敏感性。皮肤反应相关性在分离株内部（如孢子A/菌丝体A）最大，其次是特定真菌部分之间（如孢子A/孢子B），然后是分离株与部分之间（孢子A/菌丝体B）。使用附球菌皮肤反应性患者的血清，发现所有抗原的个体IgG和IgE ELISA值之间存在高度相关性。ELISA抑制结果表明，IgG识别的附球菌和链格孢抗原之间存在显著交叉反应，但IgE未识别。附球菌抗原之间的ELISA抑制交叉反应模式与皮肤反应相当，而IgG模式变化很小。在真菌不同部位提取物完全标准化之前，有必要进一步表征不同免疫球蛋白同种型之间的孢子/菌丝体和菌株间识别模式。目前，使用孢子材料进行皮肤测试和治疗附球菌敏感性似乎既为时过早又没有必要。
