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利用基质血管部分球基于免疫疗法诱导异位 T 细胞簇以增强抗肿瘤免疫。

Inducing Ectopic T Cell Clusters Using Stromal Vascular Fraction Spheroid-Based Immunotherapy to Enhance Anti-Tumor Immunity.

机构信息

Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, 03080, Korea.

Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, 03080, Korea.

出版信息

Adv Sci (Weinh). 2022 Oct;9(28):e2203842. doi: 10.1002/advs.202203842. Epub 2022 Sep 4.

DOI:10.1002/advs.202203842
PMID:36058002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9534947/
Abstract

Tertiary lymphoid structures (TLSs) provide specialized niches for immune cells, resulting in improved prognoses for patients undergoing cancer immunotherapy. Shaping TLS-like niches may improve anti-cancer immunity and overcome the current limitations of immune cell-based immunotherapy. Here, it is shown that stromal vascular fraction (SVF) from adipose tissues can enhance dendritic cell (DC)-mediated T cell immunity by inducing ectopic T lymphocyte clusters. SVF cells expanded ex vivo have phenotypes and functions similar to those of fibroblastic reticular cells in a secondary lymphoid organ, and their properties can be modulated using three-dimensional spheroid culture and coculture with DCs spiked with antigen-loaded iron oxide-zinc oxide core-shell nanoparticles. Thereby, the combination of SVF spheroids and mature DCs significantly augments T cell recruitment and retention at the injection site. This strategy elicits enhanced antigen-specific immune response and anti-tumoral immunity in mice, illustrating the potential for a novel immunotherapeutic design using SVF as a structural scaffold for TLS.

摘要

三级淋巴结构 (TLS) 为免疫细胞提供了专门的龛位，从而改善了接受癌症免疫疗法的患者的预后。塑造 TLS 样龛位可能会提高抗肿瘤免疫力并克服基于免疫细胞的免疫疗法的当前局限性。在这里，研究表明脂肪组织的基质血管部分 (SVF) 通过诱导异位 T 淋巴细胞簇来增强树突状细胞 (DC) 介导的 T 细胞免疫。体外扩增的 SVF 细胞具有类似于次级淋巴器官中纤维母细胞网状细胞的表型和功能，并且可以使用三维球体培养和与加载有抗原的负载氧化铁-氧化锌核壳纳米粒子的 DC 共培养来调节其特性。因此，SVF 球体和成熟 DC 的组合可显著增加注射部位 T 细胞的募集和保留。该策略在小鼠中引发了增强的抗原特异性免疫反应和抗肿瘤免疫力，说明了使用 SVF 作为 TLS 结构支架的新型免疫治疗设计的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/87453e6fb2fa/ADVS-9-2203842-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/fec5221e8884/ADVS-9-2203842-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/40a874f4182f/ADVS-9-2203842-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/87453e6fb2fa/ADVS-9-2203842-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/e18e2b180910/ADVS-9-2203842-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/d60f0fda1692/ADVS-9-2203842-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/4b4b823bd0bc/ADVS-9-2203842-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/4145d875187c/ADVS-9-2203842-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/fec5221e8884/ADVS-9-2203842-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/40a874f4182f/ADVS-9-2203842-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac5b/9534947/87453e6fb2fa/ADVS-9-2203842-g005.jpg

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Nature. 2022 Mar;603(7900):328-334. doi: 10.1038/s41586-022-04439-0. Epub 2022 Feb 23.
2
Tertiary lymphoid structures in cancer.癌症中的三级淋巴结构。
Science. 2022 Jan 7;375(6576):eabf9419. doi: 10.1126/science.abf9419.
3
Inducible Tertiary Lymphoid Structures: Promise and Challenges for Translating a New Class of Immunotherapy.诱导性三级淋巴结构：转化新型免疫疗法的前景与挑战。
Adv Sci (Weinh). 2025 Jan;12(1):e2406684. doi: 10.1002/advs.202406684. Epub 2024 Oct 28.
4
Tertiary lymphoid structures in diseases: immune mechanisms and therapeutic advances.疾病中的三级淋巴结构：免疫机制与治疗进展。
Signal Transduct Target Ther. 2024 Aug 28;9(1):225. doi: 10.1038/s41392-024-01947-5.
5
Tertiary lymphoid structures: new immunotherapy biomarker.三级淋巴结构：新的免疫治疗标志物。
Front Immunol. 2024 Jul 4;15:1394505. doi: 10.3389/fimmu.2024.1394505. eCollection 2024.
6
Fibril-Guided Three-Dimensional Assembly of Human Fibroblastic Reticular Cells.纤维引导的人成纤维细胞网状细胞的三维组装。
ACS Appl Bio Mater. 2024 Jun 17;7(6):3953-3963. doi: 10.1021/acsabm.4c00331. Epub 2024 May 28.
7
FRC transplantation restores lymph node conduit defects in laminin α4-deficient mice.FRC 移植可修复层粘连蛋白 α4 缺陷小鼠的淋巴结导管缺陷。
JCI Insight. 2023 Apr 24;8(8):e167816. doi: 10.1172/jci.insight.167816.
8
The cellular model for Alzheimer's disease research: PC12 cells.用于阿尔茨海默病研究的细胞模型：嗜铬细胞瘤细胞（PC12细胞）
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9
Protein Kinase Inhibitors as a New Target for Immune System Modulation and Brain Cancer Management.蛋白激酶抑制剂作为免疫系统调节和脑癌管理的新靶点。
Int J Mol Sci. 2022 Dec 10;23(24):15693. doi: 10.3390/ijms232415693.
Front Immunol. 2021 May 14;12:675538. doi: 10.3389/fimmu.2021.675538. eCollection 2021.
4
Fibroblasts as immune regulators in infection, inflammation and cancer.成纤维细胞作为感染、炎症和癌症中的免疫调节剂。
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5
Fibroblasts as a source of self-antigens for central immune tolerance.成纤维细胞作为中枢免疫耐受自身抗原的来源。
Nat Immunol. 2020 Oct;21(10):1172-1180. doi: 10.1038/s41590-020-0756-8. Epub 2020 Aug 24.
6
Cell and tissue engineering in lymph nodes for cancer immunotherapy.淋巴结中的细胞和组织工程用于癌症免疫治疗。
Adv Drug Deliv Rev. 2020;161-162:42-62. doi: 10.1016/j.addr.2020.07.023. Epub 2020 Aug 1.
7
Lymph node stromal cells: cartographers of the immune system.淋巴结基质细胞：免疫系统的绘图师。
Nat Immunol. 2020 Apr;21(4):369-380. doi: 10.1038/s41590-020-0635-3. Epub 2020 Mar 23.
8
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Nature. 2020 Jan;577(7791):549-555. doi: 10.1038/s41586-019-1922-8. Epub 2020 Jan 15.
9
Tertiary lymphoid structures improve immunotherapy and survival in melanoma.三级淋巴结构可改善黑色素瘤的免疫治疗和生存率。
Nature. 2020 Jan;577(7791):561-565. doi: 10.1038/s41586-019-1914-8. Epub 2020 Jan 15.
10
Fibroblastic reticular cells enhance T cell metabolism and survival via epigenetic remodeling.成纤维细胞网状细胞通过表观遗传重塑增强 T 细胞代谢和存活。
Nat Immunol. 2019 Dec;20(12):1668-1680. doi: 10.1038/s41590-019-0515-x. Epub 2019 Oct 21.