Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Irkutsk State Medical University, Irkutsk, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):510-513. doi: 10.1007/s10517-022-05572-6. Epub 2022 Sep 5.
Drug acetylation plays an important role in the medical practice. Modern methods of acetylation phenotype prediction are based on genotyping of polymorphisms in the second exon of the gene NAT2. Some disadvantages of these methods limit their application in the clinical practice. We developed a method of human genotyping based on identification of NAT2 gene polymorphism rs1495741 by real-time PCR. This method of genotype determination has a number of advantages: high sensitivity, simplicity, possibility of automated interpretation of the results, and feasibility in clinical laboratories.
药物乙酰化在医学实践中起着重要作用。现代乙酰化表型预测方法基于 NAT2 基因第二外显子中多态性的基因分型。这些方法的一些缺点限制了它们在临床实践中的应用。我们开发了一种基于实时 PCR 鉴定 NAT2 基因多态性 rs1495741 的人类基因分型方法。这种基因型确定方法具有许多优点：高灵敏度、简单、结果自动解释的可能性以及在临床实验室中的可行性。
