Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):490-493. doi: 10.1007/s10517-022-05567-3. Epub 2022 Sep 5.
Neural transplantation is a promising regenerative therapy in the treatment of several neurological diseases. Importantly, transplanted tissue should not become a source of pathological functional activity. To assess the possibility of maintaining the balance between excitatory and inhibitory processes, an electron microscopic immunochemical study of the GABAergic system in rat neocortical transplants was performed. Accumulation of GABA-positive label in astrocytes and a relatively insignificant immune reaction to GABA in neurons and synaptic endings were found. These findings suggest that under conditions of impaired differentiation of GABA-containing neurons that generate phasic inhibition through inhibitory synapses, tonic inhibitory influences predominate in neurotransplants due to GABA released from astrocytes.
神经移植是治疗几种神经疾病的一种有前途的再生疗法。重要的是，移植组织不应该成为病理性功能活动的来源。为了评估维持兴奋性和抑制性过程之间平衡的可能性，对大鼠新皮质移植物中的 GABA 能系统进行了电子显微镜免疫化学研究。发现 GABA 阳性标记物在星形胶质细胞中积累，而神经元和突触末梢中 GABA 的免疫反应相对不明显。这些发现表明，在 GABA 能神经元分化受损的情况下，通过抑制性突触产生相性抑制，由于星形胶质细胞释放的 GABA，神经移植物中占主导地位的是紧张性抑制影响。
