Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):454-458. doi: 10.1007/s10517-022-05586-0. Epub 2022 Sep 5.
Radioresistant sublines of non-small cell lung cancer cells differing in the p53 status were obtained: A549 (p53 wild type) and H1299 (p53 deficient). The exposure to ionizing radiation was carried out using a standard protocol developed on the basis of empirical clinical experience and consisting in exposure in a dose of 2 Gy once a day, 5 days a week up to total dose of 60 Gy. The cells survived after irradiation demonstrated reduced radiosensitivity, as well as changes in differential gene expression in comparison with parental cells. Some differences in the signaling pathways involved in DNA repair were revealed.
A549（p53 野生型）和 H1299（p53 缺失型）。采用基于经验性临床经验制定的标准方案进行电离辐射暴露，方案包括每天暴露一次 2 Gy，每周 5 天，总剂量达 60 Gy。与亲本细胞相比，照射后存活的细胞表现出辐射敏感性降低，以及差异基因表达的变化。还揭示了参与 DNA 修复的信号通路中的一些差异。
