海藻酸钠基胃黏膜保护凝胶的体外与体内有效性研究。
In Vitro and In Vivo Investigation on the Effectiveness of Alginate-Based Gastric Mucosal Protective Gel.
机构信息
National Institute for Food and Drug Control, Beijing 102629, China.
China Pharmaceutical University, Nanjing 211198, China.
出版信息
Biomed Res Int. 2022 Aug 24;2022:8287163. doi: 10.1155/2022/8287163. eCollection 2022.
OBJECTIVE
To investigate the feasibility and effectiveness of an alginate-based gastric mucosal protective gel on the gastric ulcer.
METHODS
(1) In the physical protection model, after GES-1 cell attachment add the gel to transwell chamber, add different concentrations of HCl to the gel. Absorbance was measured to assess proliferation and images of the cells migrating into the wound were taken; then the migration rate of the cells was quantified by comparing images. (2) In the gastric ulcer model, excise the gastric mucosal of SD rats; the gel and fixative were applied on the artificial ulcer immediately. Dissect rats after 10 days, and calculate the wound healing rate and analyzed histology changes.
RESULTS
The effect of hydrochloric acid on cells in the lower layer was significantly reduced after the use of gastric mucosal protection gel. The protective gel had an isolation effect on different concentrations of acid. A number of GES-1 were significantly higher than those in the control group at 24 h to 72 h ( < 0.01). The migration was observed compared with the control group. The average healing rate of ulcer in the gel group was about 50%, and the control group was about 30%. Inflammation occurred in all wound regions after ten days. In the gel group, inflammatory infiltration depth was lower than that of the control, and part of SD rats' new muscle layer appeared without inflammatory infiltration. The connective tissue proliferation promoted tissue repair. In the control group, necrosis marginal, mucosal hyperplasia, marginal lymphocyte aggregation, and bleeding were observed.
CONCLUSION
This novel gel mainly has an isolating and shielding effect to prevent the wound from being exposed to gastric acid for a long time, and it can reduce the inflammatory reaction on the wounds to promote the healing of the ulcer. The gastric mucosal protective gel cannot only promote the speed of wound healing but also improve the quality of wound healing.
目的
研究藻酸盐基胃黏膜保护凝胶对胃溃疡的可行性和有效性。
方法
（1）在物理保护模型中，GES-1 细胞附着后，将凝胶加入 Transwell 室，向凝胶中加入不同浓度的 HCl。测量吸光度以评估细胞增殖情况，并拍摄细胞迁移到伤口的图像；然后通过比较图像来量化细胞的迁移率。（2）在胃溃疡模型中，切除 SD 大鼠的胃黏膜；立即在人工溃疡上涂抹凝胶和固定剂。10 天后解剖大鼠，计算伤口愈合率并分析组织学变化。
结果
使用胃黏膜保护凝胶后，盐酸对下层细胞的作用明显减弱。保护凝胶对不同浓度的酸具有隔离作用。在 24 h 至 72 h 时，与对照组相比，GES-1 的数量明显更高（<0.01）。与对照组相比，观察到迁移。凝胶组溃疡的平均愈合率约为 50%，对照组约为 30%。十天后，所有伤口区域均出现炎症。在凝胶组中，炎症浸润深度低于对照组，部分 SD 大鼠的新肌层出现，无炎症浸润。结缔组织增生促进组织修复。在对照组中，观察到坏死边缘、黏膜增生、边缘淋巴细胞聚集和出血。
结论
这种新型凝胶主要具有隔离和屏蔽作用，可防止伤口长时间暴露于胃酸中，并能减轻伤口的炎症反应，促进溃疡愈合。胃黏膜保护凝胶不仅能促进伤口愈合速度，还能提高伤口愈合质量。
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