奥曲肽能加速大鼠慢性胃溃疡的愈合。

BACKGROUND

Previous studies have shown that administration of obestatin exhibits a protective effect in the pancreas, attenuating the development of acute pancreatitis. The aim of the present study was to investigate the influence of obestatin administration on the healing of chronic gastric ulcers.

MATERIAL/METHODS: Chronic gastric ulcers were induced in rats by 100% acetic acid applied to the serosal surface of the gastric wall. Obestatin was given twice a day intraperitoneally at the dose of 4, 8 or 16 nmol/kg/dose for 6 days. Six days after induction of ulcers, rats were anesthetized and the stomach was exposed for measurement of gastric blood flow and ulcer area. Biopsy samples from the gastric mucosa were taken for determination of mucosal DNA synthesis and for measurement of gastric expression of mRNA for interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha).

RESULTS

Induction of gastric ulcers alone increased mucosal blood flow and tissue expression of mRNA for TNF-alpha and IL-1beta, whereas gastric mucosal DNA synthesis was reduced. In rats with gastric ulcers, administration of obestatin increased gastric mucosal blood flow, accelerated the healing rate of these ulcers and partly reversed the gastric ulcer-induced reduction in gastric mucosal DNA synthesis. These results were associated with a reduction in gastric mucosal expression of pro-inflammatory IL-1beta and TNF-alpha.

CONCLUSIONS

Treatment with obestatin increases gastric mucosal blood flow and cell proliferation, leading to acceleration of healing of gastric ulcers. These effects are associated with a reduction in mucosal expression of pro-inflammatory IL-1beta and TNF-alpha.

背景

先前的研究表明，给予脑肠肽（obestatin）可在胰腺中发挥保护作用，减轻急性胰腺炎的发展。本研究旨在探讨给予脑肠肽对慢性胃溃疡愈合的影响。

材料/方法：通过将 100%乙酸应用于胃壁的浆膜表面，在大鼠中诱导慢性胃溃疡。obestatin 每天两次腹膜内给予，剂量为 4、8 或 16 nmol/kg/剂量，持续 6 天。溃疡形成后 6 天，麻醉大鼠并暴露胃以测量胃血流和溃疡面积。从胃黏膜采集活检样本，以测定黏膜 DNA 合成，并测量胃黏膜中白细胞介素 1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的 mRNA 表达。