Taylor A J, Pandov H I, Lawson N
Ann Clin Biochem. 1987 May;24 ( Pt 3):293-300. doi: 10.1177/000456328702400309.
A capillary gas liquid chromatography method was established for the routine determination of fatty acid profiles from washed erythrocyte membranes. Only the five major fatty acids found in erythrocytes (palmitic, stearic, oleic, linoleic and arachidonic acids) had acceptable precision (CVs less than 10.0%) for use in establishing quantitative differences between groups of individuals. Reference values were established for the relative amounts of these five fatty acids in adults. Significant alterations in the relative concentrations of oleic and arachidonic acids were found after storage at +4 degrees C for 24 h, which has important implications in the study of changes in erythrocyte fatty acids in cancer and diabetes.
建立了一种毛细管气液色谱法，用于常规测定洗涤红细胞膜的脂肪酸谱。红细胞中发现的仅五种主要脂肪酸（棕榈酸、硬脂酸、油酸、亚油酸和花生四烯酸）在用于建立个体组间定量差异时具有可接受的精密度（变异系数小于10.0%）。确定了成年人中这五种脂肪酸相对含量的参考值。发现在4℃储存24小时后，油酸和花生四烯酸的相对浓度有显著变化，这对癌症和糖尿病患者红细胞脂肪酸变化的研究具有重要意义。
