Michiba Ayano, Shiogama Kazuya, Tsukamoto Tetsuya, Hirayama Masaya, Yamada Seiji, Abe Masato
Department of Diagnostic Pathology, Fujita Health University Graduate School of Medicine.
Department of Morphology and Pathology, Fujita Health University Medical Science, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan.
Acta Histochem Cytochem. 2022 Aug 27;55(4):111-118. doi: 10.1267/ahc.22-00018. Epub 2022 Aug 10.
Macrophages are classified into two phenotypes, M1 and M2, based on their roles. M2 macrophages suppress inflammation and increase in proportion to the malignancy of brain tumors. Recently, macrophage extracellular traps (METs), which change into a network, have been reported as a unique form of macrophage cell death. In this study, immunohistochemical analysis of macrophages in METs in human glioblastoma was performed. To distinguish between M1 and M2 macrophages, multiple immunostainings with Iba1 combined with CD163 or CD204 were performed. M2 macrophages were present in small amounts in normal and borderline areas but showed an increasing trend as they shifted to tumor areas, and most of them were the activated- or phagocytic-type. We also successfully detected METs coexisting with fibrin and lactoferrin near the border between the tumor and necrotic area. M2 macrophages not only suppressed inflammation but also were involved in the formation of METs. This study found that M2 macrophages play various roles in unstable situations.
巨噬细胞根据其作用可分为M1和M2两种表型。M2巨噬细胞可抑制炎症，且其比例与脑肿瘤的恶性程度呈正相关。最近，已报道巨噬细胞胞外陷阱（METs）会转变为网络状，是巨噬细胞死亡的一种独特形式。在本研究中，对人胶质母细胞瘤中METs内的巨噬细胞进行了免疫组织化学分析。为区分M1和M2巨噬细胞，进行了Iba1与CD163或CD204联合的多重免疫染色。M2巨噬细胞在正常和交界区域少量存在，但随着向肿瘤区域转移呈现增加趋势，且大多数为活化型或吞噬型。我们还成功在肿瘤与坏死区域边界附近检测到与纤维蛋白和乳铁蛋白共存的METs。M2巨噬细胞不仅抑制炎症，还参与METs的形成。本研究发现M2巨噬细胞在不稳定情况下发挥多种作用。
