Momesso Santos Cesar Miguel, Amaral Jônatas Bussador, Rossi Marcelo, Vieria Rodolfo Paula, Abad Cesar Cavinato Cal, Bachi André Luis Lacerda
Interdisciplinary Post-Graduate Program in Health Sciences, Cruzeiro Do Sul University, São Paulo, Brazil.
Faculty ENAU, Ribeirão Pires, SP, Brazil.
Front Sports Act Living. 2022 Aug 17;4:915343. doi: 10.3389/fspor.2022.915343. eCollection 2022.
To investigate the effects of triathlon racing under extreme conditions on metabolic and immune/inflammatory responses.
Thirteen amateur athletes participated in an extreme triathlon competition (swim - 3.8 km; cycling - 180 km; running - 4 2 km; with a 3,700 m accumulated altitude). Blood samples were collected on three different occasions: pre-competition (baseline), immediately post-competition (IM), and 12 h post-competition (12 h) to evaluate glycemic and lipid profiles, leukocytes count, and cytokines levels in plasma and in whole-blood cell culture supernatant stimulated or not with LPS.
Decreased glucose and triglycerides levels, increased LDL, and a significant leukocytosis were observed at IM and 12 h compared to baseline. In addition, higher serum levels of IL-6, IL-8, and IL-10 were found at IM than in baseline and post-12 h. Whereas increased IL-12p40 levels were observed for 12 h compared to baseline. At baseline, in LPS-stimulated cell culture, IL-6, IL-8, and IL-12p70 were higher, while IL-12p40 levels were lower than non-stimulated cell culture. At IM, IL-12p40 levels were unchanged, while higher levels of other cytokines were found in LPS-stimulated cell culture compared to non-stimulated cell culture. The 12 h results showed higher levels of IL-6, IL-8, and IL-10 in LPS-stimulated cell culture than in non-stimulated cell culture. Additionally, a significant negative correlation between circulating glucose levels and IL-6 was found.
The triathlon competition's performance under extreme conditions has remarkable impacts on the lipid profile and systemic immune/inflammatory responses. For the first time, significant alterations in the cytokine responses of whole blood cell culture to LPS-stimulation in baseline, IM, and 12h were demonstrated.
研究极端条件下铁人三项比赛对代谢及免疫/炎症反应的影响。
13名业余运动员参加了一场极端铁人三项比赛（游泳3.8公里；自行车骑行180公里；跑步42公里；累计海拔3700米）。在三个不同时间点采集血样：比赛前（基线）、比赛结束后即刻（IM）以及比赛结束后12小时（12 h），以评估血糖和血脂谱、白细胞计数以及血浆和经或未经脂多糖（LPS）刺激的全血细胞培养上清液中的细胞因子水平。
与基线相比，在IM和12 h时观察到葡萄糖和甘油三酯水平降低、低密度脂蛋白（LDL）升高以及显著的白细胞增多。此外，IM时血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（IL-10）水平高于基线和12 h后。而与基线相比，12 h时白细胞介素-12p40水平升高。在基线时，在LPS刺激的细胞培养中，IL-6、IL-8和白细胞介素-12p70较高，而IL-12p40水平低于未刺激的细胞培养。在IM时，IL-12p40水平未改变，而与未刺激的细胞培养相比，LPS刺激的细胞培养中其他细胞因子水平较高。12 h的结果显示，LPS刺激的细胞培养中IL-6、IL-8和IL-10水平高于未刺激的细胞培养。此外，循环葡萄糖水平与IL-6之间存在显著负相关。
极端条件下的铁人三项比赛表现对血脂谱和全身免疫/炎症反应有显著影响。首次证明了在基线、IM和12 h时全血细胞培养对LPS刺激的细胞因子反应有显著改变。
