Ge Kai, Geng Zhaoyu
Department of Biological and Pharmaceutical Engineering, West Anhui University, West of Yunlu Bridge, Yu'an District, Liuan, Anhui Province, 237012, China.
Department of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province 230036, China.
Open Life Sci. 2022 Aug 17;17(1):960-972. doi: 10.1515/biol-2022-0101. eCollection 2022.
In this study, we aimed to characterize the liver protein profile of Chaohu ducks using two-dimensional electrophoresis and proteomics. The livers were quickly collected from 120 healthy, 84-day-old Chaohu ducks. The intramuscular fat (IMF) content of the left pectoralis muscle was determined using the Soxhlet extraction method. The total protein of liver tissues from the high and low IMF groups was extracted for proteomics. Functional enrichment analysis of the differentially expressed proteins (DEPs) was conducted using gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG). In total, 43 DEPs were identified. Functional enrichment analysis indicated that these DEPs were significantly related to four lipid metabolic processes: carboxylic acid metabolic process, ATP metabolic process, oxoacid metabolic process, and organic acid metabolic process. Three pathways correlated with lipid metabolism were identified using KEGG analysis: glycolysis/gluconeogenesis, pentose phosphate pathway, fructose, and mannose metabolism. Eight key proteins associated with lipid metabolism were identified: ALDOB, GAPDH, ENO1, RGN, TPI1, HSPA9, PRDX1, and GPX1. Protein-protein interaction analysis revealed that the glycolysis/gluconeogenesis pathway mediated the interaction relationship. Key proteins and metabolic pathways were closely related to lipid metabolism and showed a strong interaction in Chaohu ducks.
在本研究中，我们旨在利用二维电泳和蛋白质组学对巢湖鸭的肝脏蛋白质谱进行表征。从120只84日龄健康的巢湖鸭中快速采集肝脏。采用索氏提取法测定左胸肌的肌内脂肪（IMF）含量。提取高、低IMF组肝脏组织的总蛋白用于蛋白质组学研究。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）对差异表达蛋白（DEP）进行功能富集分析。总共鉴定出43种DEP。功能富集分析表明，这些DEP与四个脂质代谢过程显著相关：羧酸代谢过程、ATP代谢过程、含氧酸代谢过程和有机酸代谢过程。通过KEGG分析鉴定出三条与脂质代谢相关的途径：糖酵解/糖异生、磷酸戊糖途径、果糖和甘露糖代谢。鉴定出八种与脂质代谢相关的关键蛋白：醛缩酶B（ALDOB）、甘油醛-3-磷酸脱氢酶（GAPDH）、烯醇化酶1（ENO1）、视黄蛋白（RGN）、磷酸丙糖异构酶1（TPI1）、热休克蛋白9（HSPA9）、过氧化物还原酶1（PRDX1）和谷胱甘肽过氧化物酶1（GPX1）。蛋白质-蛋白质相互作用分析表明，糖酵解/糖异生途径介导了相互作用关系。关键蛋白和代谢途径与脂质代谢密切相关，在巢湖鸭中表现出强烈的相互作用。
