Li Wei-Zhen, Zhu Tong, Zhou Jing-Jiang, Shang Su-Qin
Key Laboratory of Grassland Ecosystem of Ministry of Education, and Sino-U.S. Centers for Grazingland Ecosystem Sustainability, College of Grassland Science, Gansu Agricultural University, Lanzhou, China.
College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, China.
Front Physiol. 2022 Aug 17;13:937033. doi: 10.3389/fphys.2022.937033. eCollection 2022.
To study the physiological mechanisms of in response to short-term heat stress, the eggs and the emerged adults were exposed to 38, 40, and 42°C, 85% ± 5%RH,16 h:8 h (L:D) for 2, 4, and 6 h. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) as well as the protein content of were examined. All treatments caused significant different changes compared to the untreated control. The protein content increased as the temperature increased, while it showed different changing trends with the prolongation of exposure duration. The enzymatic activity of SOD, CAT, and POD was significantly affected by the temperature treatment. Both the maximum and minimum level of the three enzymes after a short-term heat stress differed significantly to the control group ( < 0.05). The highest values of three enzymatic activities were all obtained at 40°C-4 h. Person correlation analysis indicates that the high temperature was the primary factor affecting the enzymatic activity, while the exposure duration of the heat stress was the secondary factor. In general, the short-term heat stress increased the protein content of and up-regulated the expression of SOD, CAT, and POD activities as well.
为研究[具体对象]对短期热应激的生理机制，将卵及羽化后的成虫置于38、40和42°C、相对湿度85%±5%、光照周期16小时:8小时（亮:暗）的环境中处理2、4和6小时。检测了超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性以及[具体对象]的蛋白质含量。与未处理的对照组相比，所有处理均引起了显著不同的变化。蛋白质含量随温度升高而增加，而随着暴露时间的延长呈现出不同的变化趋势。温度处理对SOD、CAT和POD的酶活性有显著影响。短期热应激后这三种酶的最高和最低水平与对照组相比均有显著差异（P<0.05）。三种酶活性的最高值均在40°C - 4小时时获得。Person相关性分析表明，高温是影响酶活性的主要因素，而热应激的暴露时间是次要因素。总体而言，短期热应激增加了[具体对象]的蛋白质含量，并上调了SOD、CAT和POD的活性表达。
