过表达超氧化物歧化酶和过氧化氢酶赋予木薯对朱砂叶螨的抗性。
Overproduction of superoxide dismutase and catalase confers cassava resistance to Tetranychus cinnabarinus.
机构信息
Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, China.
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
出版信息
Sci Rep. 2017 Jan 5;7:40179. doi: 10.1038/srep40179.
To explore the role of protective enzymes in cassava (Manihot esculenta Crantz) resistance to mites, transgenic cassava lines overproducing copper/zinc superoxide dismutase (MeCu/ZnSOD) and catalase (MeCAT1) were used to evaluate and molecularly confirm cassava resistance to Tetranychus cinnabarinus. Laboratory evaluation demonstrated that, compared with the control cultivar TMS60444 (wild type, WT), the survival, reproduction, development and activities of SOD and CAT in T. cinnabarinus feeding on transgenic cassava lines SC2, SC4, and SC11 significantly inhibited. Furthermore, the activities of SOD and CAT in transgenic cassava lines SC2, SC4, and SC11 damaged by T. cinnabarinus significantly increased. These findings were similar to the results in the mite-resistant cassava cultivars. Besides, field evaluation indicated that the transgenic cassava lines SC2, SC4, and SC11 were slightly damaged as the highly mite-resistant control C1115, while the highly mite-susceptible WT was severely damaged by T. cinnabarinus. Laboratory and field evaluation demonstrated that transgenic cassava lines were resistant to T. cinnabarinus, which directly confirmed that the increase in SOD and CAT activities was positively related to cassava resistance to T. cinnabarinus. These results will help in understanding the antioxidant defense responses in the cassava-mite interaction and molecular breeding of mite-resistant cassava for effective pest control.
为了探究保护酶在木薯（Manihot esculenta Crantz）抗螨中的作用，本研究利用过表达铜锌超氧化物歧化酶（MeCu/ZnSOD）和过氧化氢酶（MeCAT1）的转基因木薯品系来评价和分子确认木薯对朱砂叶螨（Tetranychus cinnabarinus）的抗性。实验室评估表明，与对照品种 TMS60444（野生型，WT）相比，朱砂叶螨在转基因木薯 SC2、SC4 和 SC11 上的存活率、繁殖力、发育和 SOD 和 CAT 活性均受到显著抑制。此外，SC2、SC4 和 SC11 这 3 个转基因木薯品系受朱砂叶螨侵害后的 SOD 和 CAT 活性显著增加。这些发现与抗螨木薯品种的结果相似。此外，田间评估表明，转基因木薯品系 SC2、SC4 和 SC11 受到的伤害程度与高抗螨品种 C1115 相似，而高度感螨的 WT 则受到朱砂叶螨的严重伤害。实验室和田间评估表明，转基因木薯品系对朱砂叶螨具有抗性，这直接证实了 SOD 和 CAT 活性的增加与木薯对朱砂叶螨的抗性呈正相关。这些结果有助于理解木薯-螨相互作用中的抗氧化防御反应，以及进行有效的害虫防治的抗螨木薯的分子育种。
Zotero 插件