Xpert HIV-1 病毒载量检测在塞内加尔的表现：一个 CRF02_AG 高流行地区。
Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG.
机构信息
Laboratoire de Bactériologie-Virologie CHU le Dantec, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
Clinton Health Access Initiative, Dakar, Senegal.
出版信息
Pan Afr Med J. 2022 Jun 20;42:136. doi: 10.11604/pamj.2022.42.136.32041. eCollection 2022.
INTRODUCTION
the introduction of the point-of-care in HIV-1 viral load quantification appears to be a complementary strategy to the existing conventional system of the acceleration plan for the achievement of the three 90s in Senegal. The objective of this study was to evaluate the performance of the Xpert® HIV-1 viral load in the context of circulation of non-B, non-C subtypes.
METHODS
two hundred samples, were tested on Xpert® HIV-1 Viral Load using 1 ml of plasma in comparison to 600 μl on Abbott Real-time HIV-1 assay. The difference between viral load values was considered significant for Dlog <0.5 log copies/ml.
RESULTS
a good correlation (r=0.985) was noted and confirmed using passing-bablok regression (slope 1.048; 95% CI: 1.036 to 1.069) for 188 samples with samples. A mean difference of 0.0075 log10 copies/ml for a 95% confidence interval (CI) of 0.002 log10 copies/ml to 0.013 log10 copies/ml was obtained. Sensitivity and specificity were respectively 93.6% and 93.5% at the threshold of 1.6 log10 copies/ml and 100% and 99% at the threshold of 3.0 log10 copies/ml.
CONCLUSION
these results show that Xpert® HIV-1 Viral Load has excellent performance. In Senegal, and can be used for HIV viral load monitoring.
简介
在 HIV-1 病毒载量定量方面引入即时检测似乎是塞内加尔加速实现三个 90 目标的现有常规系统的补充策略。本研究的目的是评估 Xpert® HIV-1 病毒载量在非 B、非 C 亚型流行情况下的性能。
方法
使用 1 毫升血浆对 200 个样本进行 Xpert® HIV-1 病毒载量检测，并与 Abbott Real-time HIV-1 检测法的 600μl 进行比较。对于 Dlog<0.5 log 拷贝/ml 的病毒载量值差异，认为有显著差异。
结果
对于 188 个样本，使用通过 bablok 回归（斜率 1.048；95%CI：1.036 至 1.069），注意到并证实了良好的相关性（r=0.985）。对于 95%置信区间（CI）为 0.002 log10 拷贝/ml 至 0.013 log10 拷贝/ml 的 0.0075 log10 拷贝/ml 的平均差异。在阈值为 1.6 log10 拷贝/ml 时，灵敏度和特异性分别为 93.6%和 93.5%，在阈值为 3.0 log10 拷贝/ml 时，灵敏度和特异性均为 100%和 99%。
结论
这些结果表明 Xpert® HIV-1 病毒载量具有优异的性能。在塞内加尔，可用于 HIV 病毒载量监测。
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